| Round of 64 | Lauren JonesCollege of Saint Benedict def. Lindsay SeccombeUW- Lacrosse | 8-4 |
| Round of 64 | Megan KastenUW- Whitewater def. Sarah CarlsonSt. Olaf | 8-2 |
| Round of 64 | Emily DeWittSaint Mary's University def. Kristin KiemeleHamline University | 8-6 |
| Round of 64 | W Angela ShieldsSt. Scholastica def. Ashley TrulockGustavus Adolphus College | 8-4 |
| Round of 64 | Abby MartinUW- Whitewater def. Danielle MunstermanSt. Catherine University | 8-2 |
| Round of 64 | Emily RoedigerGustavus Adolphus College def. MacKenzie RaymanCollege of Saint Benedict | 8-5 |
| Round of 64 | Andrea VivianUW- Lacrosse def. Callie SchroederSt. Norbert College | 8-5 |
| Round of 64 | Sara OhlmanCoe College def. Meghan WethingtonAugustana | 8-2 |
| Round of 64 | Crystal ZhaoUW- Whitewater def. Aimee NySt. Olaf | 8-4 |
| Round of 64 | Emily WorkcuffHamline University def. Maddie VargoMacalester | 8-3 |
| Round of 64 | Megan FallonUW- Lacrosse def. Melanie GailConcordia College | 8-2 |
| Round of 64 | Allison UtkeGustavus Adolphus College def. Megan SeipelCollege of Saint Benedict | 8-6 |
| Round of 64 | Sydney DivebissUW- Whitewater def. Abby VoigtSaint Mary's University | 8-5 |
| Round of 64 | Tricia SapioAugustana def. Lindsay GreinerWartburg College | 8-5 |
| Round of 64 | Leigh KreklauGustavus Adolphus College def. Anna KlenkeSt. Olaf | 8-2 |
| Round of 64 | Amanda O'KeefeUW- Lacrosse def. Kalyn SchroederSt. Catherine University | 8-6 |
| Round of 64 | Kelly FosterUW- Whitewater def. Elizabeth AllenHamline University | 8-3 |
| Round of 64 | Mallory ShellumGustavus Adolphus College def. Megan FialaCoe College | 8-2 |
| Round of 64 | Sarah RossmanCollege of Saint Benedict def. Ana SchmidtUW- Lacrosse | 8-4 |
| Round of 64 | Maddy BuckSt. Olaf def. Krista PankoAugustana | 8-4 |
| Round of 64 | Andrea AllisSaint Mary's University def. Heather AnnisGustavus Adolphus College | 8-3 |
| Round of 64 | Julia EllisSt. Olaf def. Jenny DallenbachWartburg College | 8-4 |
| Round of 64 | Kelly RutkowskiUW- Whitewater def. Martha GinderSt. Catherine University | 8-2 |
| Round of 64 | L Mara WeinerUW- Lacrosse def. Petra HagbergSt. Scholastica | 8-0 |
| Round of 64 | Sophie HortonGustavus Adolphus College def. Leanna JenkinsWartburg College | 8-3 |
| Round of 64 | Brittany ReeceAugustana def. Katie SchultzUW- Lacrosse | 8-5 |
| Round of 64 | Amy FreemanConcordia College def. Louise DicksonMacalester | 8-5 |
| Round of 64 | Lauren AquilarUW- Whitewater def. Ally BrandellCollege of Saint Benedict | 8-0 |
| Round of 64 | Sarah MagnusonSt. Olaf def. Marta KinsmanUW- Whitewater | 8-5 |
| Round of 64 | Amanda DlugopolskiUW- Lacrosse def. Molly YokielGustavus Adolphus College | 8-5 |
| Round of 64 | Nicole KrierHamline University def. Allyson YankleSt. Norbert College | 9-7 |
| Round of 64 | Katie RudnitskiCollege of Saint Benedict def. Ella CeizlerSt. Catherine University | 9-7 |
| Round of 32 | Lauren JonesCollege of Saint Benedict def. Megan KastenUW- Whitewater | 8-4 |
| Round of 32 | W Angela ShieldsSt. Scholastica def. Emily DeWittSaint Mary's University | 8-5 |
| Round of 32 | Abby MartinUW- Whitewater def. Emily RoedigerGustavus Adolphus College | 8-6 |
| Round of 32 | Andrea VivianUW- Lacrosse def. Sara OhlmanCoe College | 8-5 |
| Round of 32 | Crystal ZhaoUW- Whitewater def. Emily WorkcuffHamline University | 8-0 |
| Round of 32 | Allison UtkeGustavus Adolphus College def. Megan FallonUW- Lacrosse | 8-6 |
| Round of 32 | Sydney DivebissUW- Whitewater def. Tricia SapioAugustana | 8-1 |
| Round of 32 | Amanda O'KeefeUW- Lacrosse def. Leigh KreklauGustavus Adolphus College | 8-6 |
| Round of 32 | Mallory ShellumGustavus Adolphus College def. Kelly FosterUW- Whitewater | 9-7 |
| Round of 32 | Sarah RossmanCollege of Saint Benedict def. Maddy BuckSt. Olaf | 9-8 |
| Round of 32 | Andrea AllisSaint Mary's University def. Julia EllisSt. Olaf | 8-5 |
| Round of 32 | Mara WeinerUW- Lacrosse def. Kelly RutkowskiUW- Whitewater | 8-2 |
| Round of 32 | Sophie HortonGustavus Adolphus College def. Brittany ReeceAugustana | 8-5 |
| Round of 32 | Lauren AquilarUW- Whitewater def. Amy FreemanConcordia College | 8-2 |
| Round of 32 | Amanda Dlugopolski def. Sarah MagnusonSt. Olaf | 8-4 |
| Round of 32 | Katie RudnitskiCollege of Saint Benedict def. Nicole KrierHamline University | 8-6 |
| Round of 16 | L Lauren JonesCollege of Saint Benedict def. Angela ShieldsSt. Scholastica | 6-2, 0-6, 10-8 |
| Round of 16 | Andrea VivianUW- Lacrosse def. Abby MartinUW- Whitewater | 9-8 |
| Round of 16 | Allison UtkeGustavus Adolphus College def. Crystal ZhaoUW- Whitewater | 8-6 |
| Round of 16 | Amanda O'KeefeUW- Lacrosse def. Sydney DivebissUW- Whitewater | 8-6 |
| Round of 16 | Mallory ShellumGustavus Adolphus College def. Sarah RossmanCollege of Saint Benedict | 8-2 |
| Round of 16 | Mara WeinerUW- Lacrosse def. Andrea AllisSaint Mary's University | 8-2 |
| Round of 16 | Lauren AquilarUW- Whitewater def. Sophie HortonGustavus Adolphus College | 8-2 |
| Round of 16 | Amanda DlugopolskiUW- Lacrosse def. Katie RudnitskiCollege of Saint Benedict | 8-3 |
| Quarterfinal | Andrea VivianUW- Lacrosse def. Lauren JonesCollege of Saint Benedict | 6-3, 1-6, 10-4 |
| Quarterfinal | Sydney DivebissUW- Whitewater def. Allison UtkeGustavus Adolphus College | 6-2, 6-1 |
| Quarterfinal | Mallory ShellumGustavus Adolphus College def. Mara WeinerUW- Lacrosse | 6-0, 6-4 |
| Quarterfinal | Lauren AquilarUW- Whitewater def. Amanda DlugopolskiUW- Lacrosse | 6-1, 6-0 |
| Semifinal | Andrea VivianUW- Lacrosse def. Sydney DivebissUW- Whitewater | 7-6 (0-4), 6-2 |
| Semifinal | Lauren AquilarUW- Whitewater def. Mallory ShellumGustavus Adolphus College | 7-6 (0-6), 6-0 |
| Final | Lauren AquilarUW- Whitewater def. Andrea VivianUW- Lacrosse | 6-2, 6-0 |
