| Play-in | Amanda WenzelMacalester def. Sarah SchoenwalderUW- Lacrosse | 6-0, 6-2 |
| Play-in | Frances LeungCarleton College def. Erika ZevinBeloit College | 6-0, 6-1 |
| Play-in | Kelly StadumUW- Lacrosse def. Kylie HuetherCollege of Saint Benedict | 6-1, 6-1 |
| Round of 64 | Kathryn SchmidtCarleton College def. Jenna RichardsonAugustana | 6-0, 6-0 |
| Round of 64 | L Hollis McPeekCarthage def. Milly Che LeonSt. Scholastica | by default |
| Round of 64 | Anna SmithUW- Whitewater def. Katie EdinConcordia College | 6-3, 6-2 |
| Round of 64 | Katie AllenGustavus Adolphus College def. Emily HeyingWartburg College | 6-4, 4-6, 10-5 |
| Round of 64 | Rachel TiegsCollege of Saint Benedict def. Katy DelagardelleCoe College | 6-3, 4-6, 11-9 |
| Round of 64 | Michaela BurroughsSt. Catherine University def. Abby WerwieUW-Stout | 6-2, 6-1 |
| Round of 64 | Kelly KuetherUW- Lacrosse def. Christine MullerSt. Olaf | 6-4, 7-5 |
| Round of 64 | Marianne BarauGustavus Adolphus College def. Lauren PostSt. Norbert College | 6-3, 4-6, 12-10 |
| Round of 64 | Hannah JensenCoe College def. Mary RoopGustavus Adolphus College | 6-2, 6-3 |
| Round of 64 | Rebecca SnyderSaint Mary's University def. Amy SiemonUW- Whitewater | 7-6 (6-0), 6-1 |
| Round of 64 | Ali OnealGustavus Adolphus College def. Amanda WenzelMacalester | 6-1, 6-1 |
| Round of 64 | Caitlyn HarperSt. Olaf def. Tricia CrawfordAugustana | 6-3, 7-6, 6-4 |
| Round of 64 | Michelle DaciolasUW- Whitewater def. Gina KleinCarthage | 6-4, 6-7 (0-3), 10-5 |
| Round of 64 | Jenny MarayamaUW- Lacrosse def. Kelsey EathersonHamline University | 6-3, 6-1 |
| Round of 64 | Beth LarsonLawrence def. Maria BryanGustavus Adolphus College | 7-6 (0-4), 6-7 (0-5), 10-6 |
| Round of 64 | Frances LeungCAR def. Leanna NelsonWartburg College | 6-2, 6-1 |
| Round of 64 | Hillary AllenCoe College def. Kristina NaidiczCarthage | 6-4, 6-3 |
| Round of 64 | Allie HinmanUW-Stout def. Carly BuhrmanUW- Lacrosse | 6-2, 6-2 |
| Round of 64 | Alex EricksonGustavus Adolphus College def. Kathleen KappelUW- Whitewater | 6-4, 6-2 |
| Round of 64 | Molly EslickWartburg College def. Kendra AcklandSt. Catherine University | 6-3, 6-3 |
| Round of 64 | Bridget DoyleCarleton College def. Claire CarlsonSt. Olaf | 6-1, 6-1 |
| Round of 64 | Ingrid StensvaagUW- Whitewater def. Kelly StadumUW- Lacrosse | 6-7 (0-2), 6-3, 11-9 |
| Round of 64 | Paige SchaeferMacalester def. Anna YarboroughHamline University | 3-6, 6-1, 11-9 |
| Round of 64 | Sierra KrebsbachGustavus Adolphus College def. Michelle SchupbachCoe College | 6-1, 6-1 |
| Round of 64 | Sam FrankGustavus Adolphus College def. Stephanie LeeMacalester | 6-0, 6-3 |
| Round of 64 | L Beth KaufmanCarthage def. Callee CondaSt. Scholastica | 6-1, 6-2 |
| Round of 64 | Erin GudulCarleton College def. Katie McDonaldUW- Lacrosse | 6-2, 7-5 |
| Round of 64 | Andrea SchupbachCoe College def. Hannah PerwinBeloit College | 7-5, 6-3 |
| Round of 64 | Megan GaardGustavus Adolphus College def. Jennifer RoeschLawrence | 6-2, 7-6 (0-2) |
| Round of 64 | Bailey EdwardsSaint Mary's University def. Abby HoSt. Olaf | 6-2, 7-5 |
| Round of 64 | Andrea RognlienConcordia College def. Jenna OlsonSt. Catherine University | 6-2, 6-1 |
| Round of 64 | Kelly Van HandelSt. Norbert College def. Kalla SchaeferUW- Whitewater | 6-1, 6-3 |
| Round of 32 | Kathryn SchmidtCarleton College def. Hollis McPeekCarthage | 6-2, 6-1 |
| Round of 32 | Anna SmithUW- Whitewater def. Katie AllenGustavus Adolphus College | 6-4, 5-7, 10-8 |
| Round of 32 | Rachel TiegsCollege of Saint Benedict def. Michaela BurroughsSt. Catherine University | 6-3, 7-6 (0-2) |
| Round of 32 | Marianne BarauGustavus Adolphus College def. Kelly KuetherUW- Lacrosse | 7-5, 3-6, 16-14 |
| Round of 32 | Hannah JensenCoe College def. Rebecca SnyderSaint Mary's University | 4-6, 6-3, 10-6 |
| Round of 32 | Ali OnealGustavus Adolphus College def. Caitlyn HarperSt. Olaf | 6-0, 6-1 |
| Round of 32 | Michelle DaciolasUW- Whitewater def. Jenny MarayamaUW- Lacrosse | 6-3, 6-1 |
| Round of 32 | Frances LeungCarleton College def. Beth LarsonLawrence | 1-6, 6-4, 10-6 |
| Round of 32 | Allie HinmanUW-Stout def. Hillary AllenCoe College | 6-1, 6-1 |
| Round of 32 | Molly EslickWartburg College def. Alex EricksonGustavus Adolphus College | 6-4, 6-4 |
| Round of 32 | Bridget DoyleCarleton College def. Ingrid StensvaagUW- Whitewater | 4-6, 6-2, 10-6 |
| Round of 32 | Sierra KrebsbachGustavus Adolphus College def. Paige SchaeferMacalester | 6-1, 6-0 |
| Round of 32 | Sam FrankGustavus Adolphus College def. Beth KaufmanCarthage | 6-1, 7-6 (0-1) |
| Round of 32 | Andrea SchupbachCoe College def. Erin GudulCarleton College | 7-5, 6-3 |
| Round of 32 | Bailey EdwardsSaint Mary's University def. Megan GaardGustavus Adolphus College | 6-3, 1-6, 10-5 |
| Round of 32 | Andrea RognlienConcordia College def. Kalla SchaeferUW- Whitewater | 7-6 (0-3), 7-5 |
| Round of 16 | Kathryn SchmidtCarleton College def. Anna SmithUW- Whitewater | 6-2, 7-5 |
| Round of 16 | Marianne BarauGustavus Adolphus College def. Rachel TiegsCollege of Saint Benedict | 6-1, 6-4 |
| Round of 16 | Ali O'nealGustavus Adolphus College def. Hannah JensenCoe College | 6-4, 6-2 |
| Round of 16 | Michelle DaciolasUW- Whitewater def. Frances LeungCarleton College | 6-3, 6-4 |
| Round of 16 | Molly EslickWartburg College def. Allie HinmanUW-Stout | 6-1, 6-1 |
| Round of 16 | Sierra KrebsbachGustavus Adolphus College def. Bridget DoyleCarleton College | 6-1, 5-7, 6-4 |
| Round of 16 | Sam FrankGustavus Adolphus College def. Andrea SchupbachCoe College | 6-0, 6-0 |
| Round of 16 | Bailey EdwardsSaint Mary's University def. Andrea RognlienConcordia College | 6-3, 6-0 |
| Quarterfinal | Kathryn SchmidtCarleton College def. Marianne BarauGustavus Adolphus College | 6-1, 6-1 |
| Quarterfinal | Ali O'nealGustavus Adolphus College def. Michelle DaciolasUW- Whitewater | 6-1, 6-0 |
| Quarterfinal | Sierra KrebsbachGustavus Adolphus College def. Molly EslickWartburg College | 6-2, 7-5 |
| Quarterfinal | Sam FrankGustavus Adolphus College def. Bailey EdwardsSaint Mary's University | 6-3, 6-0 |
| Semifinal | Sam FrankGustavus Adolphus College def. Sierra KrebsbachGustavus Adolphus College | 3-6, 6-4, 6-2 |
| Semifinal | Kathryn SchmidtCarleton College def. Ali O'nealGustavus Adolphus College | 6-0, 7-5 |
| Final | Kathryn SchmidtCarleton College def. Sam FrankGustavus Adolphus College | 6-4, 6-1 |
| Round of 64 | vs. | no result |
| Round of 64 | vs. | no result |