| Round of 64 | Hannah JensenCoe College def. BYE | by default |
| Round of 64 | Kelly StadumUW-La Crosse def. Megan KastenUW-Whitewater | 6-2, 6-2 |
| Round of 64 | Allie HinmanUW-Stout def. Elise AllenLuther College | 5-7, 6-2, 7-5 |
| Round of 64 | Julie BardenwerperGustavus Adolphus College def. Gayatri MalhotraLawrence University | 6-0, 7-5 |
| Round of 64 | Molly EslickWartburg College def. Rebecca HeczkoMarian University | 6-0, 6-0 |
| Round of 64 | Brynne ZinneckerLuther College def. Morgan CountsGrinnell College | 7-6 (7-5), 6-2 |
| Round of 64 | Hollis McPeekCarthage College def. Killian EdwardsSaint Mary's University | 6-0, 6-1 |
| Round of 64 | Alex EricksonGustavus Adolphus College def. Kristen KiemeleHamline University | 6-3, 7-5 |
| Round of 64 | Bailey EdwardsSaint Mary's University def. Michaela BurroughsSt. Catherine's University | 6-4, 6-0 |
| Round of 64 | Katie McDonaldUW-La Crosse def. Emily RoedigerGustavus Adolphus College | 6-3, 7-5 |
| Round of 64 | Alana HumpalLuther College def. Toby CainGrinnell College | 6-3, 6-2 |
| Round of 64 | Annie SmithUW-Whitewater def. Sara OhlmanCoe College | 6-1, 6-0 |
| Round of 64 | Anne LombardiCarleton College def. Elizabeth LarsonLawrence University | 6-3, 1-6, 6-3 |
| Round of 64 | Lauren AguilarUW-Whitewater def. Amber HempEdgewood College | 6-4, 6-0 |
| Round of 64 | Sam FrankGustavus Adolphus College def. Mara WeinerUW-La Crosse | 6-1, 6-0 |
| Round of 64 | W Callee CondaSt. Scholastica def. Anna YarbroughHamline University | 7-5, 6-2 |
| Round of 64 | Jenny MaruyamaUW-La Crosse def. Shae BeckwithEdgewood College | 6-1, 6-1 |
| Round of 64 | L Megan GaardGustavus Adolphus College def. Hillary BungardenSt. Scholastica | 6-0, 6-0 |
| Round of 64 | Cass ChalhoubLuther College def. Kelsey EathertonHamline University | 6-0, 6-0 |
| Round of 64 | Michelle SchupbachCoe College def. Alexandra BaylissUW-Whitewater | 6-4, 6-1 |
| Round of 64 | Bari ReynoldsCarthage College def. Michelle ZwickyMarian University | 6-0, 6-2 |
| Round of 64 | Fran FairfieldLuther College def. Abbie WerwieUW-Stout | 6-2, 6-3 |
| Round of 64 | Jennifer DallenbachWartburg College def. Constanza AlarconGrinnell College | 6-2, 6-1 |
| Round of 64 | Abby HoSt. Olaf def. Sarah ThappaCarleton College | 6-3, 6-2 |
| Round of 64 | Clothilde ThirouinGrinnell College def. Jennifer RoeschLawrence University | 6-2, 6-1 |
| Round of 64 | Caitlin HarperSt. Olaf def. Amy SiemonUW-Whitewater | 6-2, 6-4 |
| Round of 64 | Tai LuceroCoe College def. Becca SnyderSaint Mary's University | 6-1, 6-1 |
| Round of 64 | Maria BryanGustavus Adolphus College def. Bridget DoyleCarleton College | 6-3, 3-6, 6-3 |
| Round of 64 | W Danni SonnenfeldSt. Scholastica def. Lauren BeadnellSt. Catherine's University | 3-6, 6-3, 6-4 |
| Round of 64 | Kelly KuetherUW-La Crosse def. Leanna NelsonWartburg College | 6-1, 6-4 |
| Round of 64 | Syd DivelbissUW-Whitewater def. Kristina NaidiczCarthage College | 6-0, 6-1 |
| Round of 64 | Marianne BarauGustavus Adolphus College def. Hannah PinecsakEdgewood College | 6-0, 6-1 |
| Round of 32 | Hannah JensenCoe College def. Kelly StadumUW-La Crosse | 7-6 (7-5), 6-3 |
| Round of 32 | Allie HinmanUW-Stout def. Julie BardenwerperGustavus Adolphus College | 6-2, 6-1 |
| Round of 32 | Molly EslickWartburg College def. Brynne ZinneckerLuther College | 6-2, 6-2 |
| Round of 32 | Alex EricksonGustavus Adolphus College def. Hollis McPeekCarthage College | 6-2, 6-2 |
| Round of 32 | Bailey EdwardsSaint Mary's University def. Katie McDonaldUW-La Crosse | 6-2, 6-4 |
| Round of 32 | Alana HumpalLuther College def. Annie SmithUW-Whitewater | 7-6 (7-5), 6-2 |
| Round of 32 | Anne LombardiCarleton College def. Lauren AguilarUW-Whitewater | 6-2, 6-1 |
| Round of 32 | L Sam FrankGustavus Adolphus College def. Callee CondaSt. Scholastica | 6-3, 6-0 |
| Round of 32 | Megan GaardGustavus Adolphus College def. Jenny MaruyamaUW-La Crosse | 6-2, 6-2 |
| Round of 32 | Michelle SchupbachCoe College def. Cass ChalhoubLuther College | 6-2, 6-0 |
| Round of 32 | Fran FairfieldLuther College def. Bari ReynoldsCarthage College | 6-0, 6-1 |
| Round of 32 | Abby HoSt. Olaf def. Jennifer DallenbachWartburg College | 6-1, 6-0 |
| Round of 32 | Caitlin HarperSt. Olaf def. Clothilde ThirouinGrinnell College | 4-6, 6-1, 7-6 (7-2) |
| Round of 32 | Maria BryanGustavus Adolphus College def. Tai LuceroCoe College | 6-3, 6-4 |
| Round of 32 | L Kelly KuetherUW-La Crosse def. Danni SonnenfeldSt. Scholastica | 6-2, 6-1 |
| Round of 32 | Marianne BarauGustavus Adolphus College def. Syd DivelbissUW-Whitewater | 6-1, 6-3 |
| Round of 16 | Hannah JensenCoe College def. Allie HinmanUW-Stout | 6-2, 6-4 |
| Round of 16 | Alex EricksonGustavus Adolphus College def. Molly EslickWartburg College | 6-3, 6-4 |
| Round of 16 | Bailey EdwardsSaint Mary's University def. Alana HumpalLuther College | 6-1, 7-6 (8-6) |
| Round of 16 | Sam FrankGustavus Adolphus College def. Anne LombardiCarleton College | 6-3, 1-6, 10-8 |
| Round of 16 | Megan GaardGustavus Adolphus College def. Michelle SchupbachCoe College | 6-2, 7-6 (7-4) |
| Round of 16 | Fran FairfieldLuther College def. Abby HoSt. Olaf | 7-6 (7-4), 3-6, 10-5 |
| Round of 16 | Maria BryanGustavus Adolphus College def. Caitlin HarperSt. Olaf | 6-4, 6-2 |
| Round of 16 | Marianne BarauGustavus Adolphus College def. Kelly KuetherUW-La Crosse | 6-1, 6-2 |
| Quarterfinal | Megan GaardGustavus Adolphus College def. Fran FairfieldLuther College | 6-3, 6-4 |
| Quarterfinal | Marianne BarauGustavus Adolphus College def. Maria BryanGustavus Adolphus College | 6-3, 6-3 |
| Quarterfinal | Hannah JensenCoe College def. Alex EricksonGustavus Adolphus College | 4-6, 6-3, 11-9 |
| Quarterfinal | Sam FrankGustavus Adolphus College def. Bailey EdwardsSaint Mary's University | 7-6 (7-3), 6-3 |
| Semifinal | Megan GaardGustavus Adolphus College def. Marianne BarauGustavus Adolphus College | 6-3, 5-7, 6-2 |
| Semifinal | Sam FrankGustavus Adolphus College def. Hannah JensenCoe College | 6-4, 6-3 |
| Final | Sam FrankGustavus Adolphus College def. Megan GaardGustavus Adolphus College | 6-4, 6-3 |
| Consolation | W Hillary BungardenSt. Scholastica def. Shae BeckwithEdgewood College | 6-2, 5-7, 1-0 (10-5) |
| Consolation | L Alexandra BaylissUW-Whitewater def. Hillary BungardenSt. Scholastica | by default |
