| Round of 128 | Megan JansGrinnell College def. Shanna MogdenCarroll University | 6-3, 7-6 (5-0) |
| Round of 128 | Kendra WoyahnUW-La Crosse def. Lilly HartmanGustavus Adolphus | 6-3, 7-6 (4-0) |
| Round of 128 | Paige KuepersUW-Eau Claire def. Libby ProtzEdgewood College | 6-2, 6-2 |
| Round of 128 | Katlyn GahanSt. Norbert College def. Maya MacGibbonSt. Olaf | 6-1, 6-0 |
| Round of 128 | Taylor BolyardCarthage def. Karianna GrindbergCentral | 6-1, 6-1 |
| Round of 128 | Kendra BerryUW-Eau Claire def. Alicia KnollSt. Catherine University | 7-5, 5-7, 10-5 |
| Round of 128 | Ally BakerGustavus Adolphus def. Anushka JoshiGrinnell College | 6-0, 6-0 |
| Round of 128 | Ashley MillerWis.-Whitewater def. Lauren KellerSt. Thomas | 6-4, 6-4 |
| Round of 128 | Jackie CychoszLuther def. Audra HoffmanSt. Thomas | 2-6, 6-1, 10-4 |
| Round of 128 | L Katherine AkeSt. Norbert College def. Daniela RojasSt. Scholastica | 6-2, 6-3 |
| Round of 128 | Alicia BeckWis.-Whitewater def. Sophie RobertsonCornell College | 6-1, 6-4 |
| Round of 128 | Megan KrzewinskiCarroll University def. Della BrownMacalester | 6-0, 6-1 |
| Round of 128 | Colleen McCulloughUW-Eau Claire def. Emily ButlerHamline University | 6-0, 6-0 |
| Round of 128 | Taylor ShortlyGustavus Adolphus def. Virginia PurcellGrinnell College | 6-2, 6-0 |
| Round of 128 | Jillian WallaceWis.-Whitewater def. Laura SondaySaint Mary's University | 6-0, 6-1 |
| Round of 128 | Ashley SolbergConcordia Wisconsin def. Jordan DanielsonHamline University | 6-4, 6-4 |
| Round of 128 | Cal CarlsonGustavus Adolphus def. Saniya RanaMacalester | 6-2, 6-2 |
| Round of 128 | W Jessica SolbergSt. Scholastica def. Anna GoszSt. Norbert College | 7-5, 6-3 |
| Round of 128 | Julia KrumholzCarleton College def. Megan DavisCentral | 6-3, 6-2 |
| Round of 128 | Lauren WilliamsSt. Thomas def. Amelia CoganGrinnell College | 1-0, 1-0 |
| Round of 128 | Margaret ZimmermanSt. Olaf def. Gabriella FerroCornell College | 6-2, 6-2 |
| Round of 128 | Brianna HartmannGustavus Adolphus def. Lauren WelchLuther | 6-3, 6-1 |
| Round of 128 | Shashanna MollCornell College def. Maddie MuotkaHamline University | 6-0, 6-1 |
| Round of 128 | Angie LorenzenSt. Catherine University def. Brooke VolponeUW-Eau Claire | 6-7 (0-9), 6-4, 10-5 |
| Round of 128 | Diannah WatsonCoe College def. Jenna ZimmermanSt. Thomas | 6-7 (0-9), 7-5, 13-11 |
| Round of 128 | Katie AneyGustavus Adolphus def. Morgan WilsonEdgewood College | 6-0, 7-6 (2-0) |
| Round of 128 | Laura BirkyCoe College def. Ashley MaleyCentral | 4-6, 6-2, 10-6 |
| Round of 128 | Olivia GallagherUW-Eau Claire def. Judith FanGrinnell College | 7-6 (4-0), 7-5 |
| Round of 128 | Rachel SiemonWis.-Whitewater def. Jessie O'BrienSt. Thomas | 6-2, 6-1 |
| Round of 64 | Megan HumphreysWis.-Whitewater def. Megan JansGrinnell College | 6-0, 6-0 |
| Round of 64 | Kendra WoyahnUW-La Crosse def. Paige KuepersUW-Eau Claire | 6-3, 6-4 |
| Round of 64 | Maya MacGibbonSt. Olaf def. Maggie HelmsLuther | 6-2, 6-1 |
| Round of 64 | Caitlin BakkeSt. Thomas def. Sarah LaseckiCoe College | 6-0, 6-3 |
| Round of 64 | Karen MoeNorthwestern (Minn.) def. Sadie SpahnUW-La Crosse | 4-6, 6-4, 10-7 |
| Round of 64 | Kendra BerryUW-Eau Claire def. Taylor BolyardCarthage | 5-7, 7-5, 10-5 |
| Round of 64 | Danielle VasilievCarleton College def. Ally BakerGustavus Adolphus | 6-2, 6-4 |
| Round of 64 | Julia ThomeCornell College def. Ashley MillerWis.-Whitewater | 6-4, 7-6 (2-0) |
| Round of 64 | Michaela SchulzGustavus Adolphus def. Jackie CychoszLuther | 6-0, 7-6 (3-0) |
| Round of 64 | Angie AllgoodCentral def. Tamara WashingtonCoe College | 6-3, 6-1 |
| Round of 64 | Kimmy MrozekUW-La Crosse def. Kirsten HoffmannSaint Mary's University | 6-2, 6-2 |
| Round of 64 | Alexandra TalyanskyWis.-Whitewater def. Katherine AkeSt. Norbert College | 6-1, 6-0 |
| Round of 64 | Shelby HendersonSt. Thomas def. Alicia BeckWis.-Whitewater | 6-3, 5-7, 10-8 |
| Round of 64 | Megan KrzewinskiCarroll University def. Hannah TresedderConcordia Wisconsin | 7-6 (5-0), 3-6, 19-17 |
| Round of 64 | Amy SebastianCoe College def. Colleen McCulloughUW-Eau Claire | 6-1, 6-2 |
| Round of 64 | Max MellaCarthage def. Taylor ShortlyGustavus Adolphus | 6-3, 6-3 |
| Round of 64 | Jillian WallaceWis.-Whitewater def. Sarah HendersonUW-Eau Claire | 6-1, 7-5 |
| Round of 64 | Mikinzee SaloSt. Catherine University def. Ashley SolbergConcordia Wisconsin | 6-1, 6-1 |
| Round of 64 | Cal CarlsonGustavus Adolphus def. Lauren MiketinacCarroll University | 5-7, 6-4, 10-5 |
| Round of 64 | L Bridget NoackSt. Thomas def. Jessica SolbergSt. Scholastica | 6-0, 6-0 |
| Round of 64 | Sid DirksGustavus Adolphus def. Anna MilesEdgewood College | 4-6, 6-1, 10-5 |
| Round of 64 | Haley ResnickCoe College def. Julia KrumholzCarleton College | 6-0, 6-1 |
| Round of 64 | Margaret ZimmermanSt. Olaf def. Lauren WilliamsSt. Thomas | 6-2, 6-4 |
| Round of 64 | Gabi KitchellWis.-Whitewater def. Eliza BarterCarthage | 6-0, 6-1 |
| Round of 64 | Brianna HartmannGustavus Adolphus def. Elizabeth ManlickSt. Norbert College | 1-0, 1-0 |
| Round of 64 | Alisa HallSt. Olaf def. Shashanna MollCornell College | 6-0, 6-0 |
| Round of 64 | Angie LorenzenSt. Catherine University def. Lily HamiltonGrinnell College | 4-6, 6-1, 10-5 |
| Round of 64 | Bridgid McGuireWis.-Whitewater def. Diannah WatsonCoe College | 6-1, 6-1 |
| Round of 64 | Katie AneyGustavus Adolphus def. Casey FrommerCarthage | 6-4, 6-1 |
| Round of 64 | Mikayla BecichCarleton College def. Katherine RossWartburg College | 6-3, 6-0 |
| Round of 64 | Olivia GallagherUW-Eau Claire def. Laura BirkyCoe College | 5-7, 7-5, 10-6 |
| Round of 64 | Bridget BellissimoUW-La Crosse def. Rachel SiemonWis.-Whitewater | 6-0, 6-1 |
| Round of 32 | Megan HumphreysWis.-Whitewater def. Kendra WoyahnUW-La Crosse | 6-0, 6-1 |
| Round of 32 | Maya MacGibbonSt. Olaf def. Caitlin BakkeSt. Thomas | 6-2, 2-6, 10-6 |
| Round of 32 | Karen MoeNorthwestern (Minn.) def. Taylor BolyardCarthage | 6-1, 6-1 |
| Round of 32 | Danielle VasilievCarleton College def. Julia ThomeCornell College | 3-6, 6-2, 10-6 |
| Round of 32 | Michaela SchulzGustavus Adolphus def. Angie AllgoodCentral | 6-1, 6-0 |
| Round of 32 | Alexandra TalyanskyWis.-Whitewater def. Kimmy MrozekUW-La Crosse | 6-3, 6-4 |
| Round of 32 | Megan KrzewinskiCarroll University def. Shelby HendersonSt. Thomas | 6-7 (0-7), 6-2, 10-5 |
| Round of 32 | Amy SebastianCoe College def. Max MellaCarthage | 6-4, 6-4 |
| Round of 32 | Jillian WallaceWis.-Whitewater def. Mikinzee SaloSt. Catherine University | 2-6, 6-4, 10-6 |
| Round of 32 | Bridget NoackSt. Thomas def. Cal CarlsonGustavus Adolphus | 6-3, 6-2 |
| Round of 32 | Haley ResnickCoe College def. Sid DirksGustavus Adolphus | 1-6, 7-5, 10-4 |
| Round of 32 | Gabi KitchellWis.-Whitewater def. Margaret ZimmermanSt. Olaf | 6-2, 6-0 |
| Round of 32 | Alisa HallSt. Olaf def. Brianna HartmannGustavus Adolphus | 6-2, 6-1 |
| Round of 32 | Bridgid McGuireWis.-Whitewater def. Angie LorenzenSt. Catherine University | 6-2, 6-0 |
| Round of 32 | Katie AneyGustavus Adolphus def. Mikayla BecichCarleton College | 7-6 (4-0), 6-2 |
| Round of 32 | Bridget BellissimoUW-La Crosse def. Olivia GallagherUW-Eau Claire | 6-0, 6-1 |
| Round of 16 | Megan HumphreysWis.-Whitewater def. Maya MacGibbonSt. Olaf | 6-0, 6-0 |
| Round of 16 | Karen MoeNorthwestern (Minn.) def. Taylor BolyardCarthage | 6-2, 6-4 |
| Round of 16 | Michaela SchulzGustavus Adolphus def. Alexandra TalyanskyWis.-Whitewater | 6-4, 6-2 |
| Round of 16 | Amy SebastianCoe College def. Megan KrzewinskiCarroll University | 6-0, 6-0 |
| Round of 16 | Bridget NoackSt. Thomas def. Jillian WallaceWis.-Whitewater | 6-2, 7-6 (8-0) |
| Round of 16 | Gabi KitchellWis.-Whitewater def. Haley ResnickCoe College | 6-2, 6-1 |
| Round of 16 | Alisa HallSt. Olaf def. Bridgid McGuireWis.-Whitewater | 7-6 (8-0), 6-1 |
| Round of 16 | Katie AneyGustavus Adolphus def. Bridget BellissimoUW-La Crosse | 6-1, 6-0 |
| Quarterfinal | Megan HumphreysWis.-Whitewater def. Karen MoeNorthwestern (Minn.) | 6-1, 6-3 |
| Quarterfinal | Michaela SchulzGustavus Adolphus def. Amy SebastianCoe College | 6-2, 7-6 (4-0) |
| Quarterfinal | Gabi KitchellWis.-Whitewater def. Bridget NoackSt. Thomas | 6-1, 6-2 |
| Quarterfinal | Bridget BellissimoUW-La Crosse def. Alisa HallSt. Olaf | 6-3, 6-1 |
| Semifinal | Megan HumphreysWis.-Whitewater def. Michaela SchulzGustavus Adolphus | 2-6, 7-6 (5-0), 6-1 |
| Semifinal | Gabi KitchellWis.-Whitewater def. Bridget BellissimoUW-La Crosse | 6-3, 5-7, 6-3 |
| Final | Megan HumphreysWis.-Whitewater def. Gabi KitchellWis.-Whitewater | 7-5, 6-0 |
| Consolation final | Lilly HartmanGustavus Adolphus def. Libby ProtzEdgewood College | 8-0 |
| Consolation final | Maggie HelmsLuther def. Katlyn GahanSt. Norbert College | 8-2 |
| Consolation final | Hannah TresedderConcordia Wisconsin def. Della BrownMacalester | 6-2, 7-5 |
| Consolation final | Alicia KnollSt. Catherine University def. Karianna GrindbergCentral | 6-1, 7-5 |
| Consolation final | Sarah HendersonUW-Eau Claire def. Laura SondaySaint Mary's University | 8-3 |
| Consolation final | Lauren MiketinacCarroll University def. Saniya RanaMacalester | 8-0 |
| Consolation final | Elizabeth ManlickSt. Norbert College def. Lauren WelchLuther | 8-1 |
| Consolation final | Brooke VolponeUW-Eau Claire def. Lily HamiltonGrinnell College | 8-6 |
| Consolation final | Casey FrommerCarthage def. Morgan WilsonEdgewood College | 6-1, 6-2 |
| Consolation final | Lilly HartmanGustavus Adolphus def. Shanna MogdenCarroll University | 6-3, 6-3 |
| Consolation final | Maggie HelmsLuther def. Sarah LaseckiCoe College | 6-4, 6-3 |
| Consolation final | Sadie SpahnUW-La Crosse def. Alicia KnollSt. Catherine University | 6-2, 6-1 |
| Consolation final | Anushka JoshiGrinnell College def. Lauren KellerSt. Thomas | 1-0, 1-0 |
| Consolation final | Tamara WashingtonCoe College def. Audra HoffmanSt. Thomas | 1-0, 1-0 |
| Consolation final | W Daniela RojasSt. Scholastica def. Kirsten HoffmannSaint Mary's University | 8-4 |
| Consolation final | Sophie RobertsonCornell College def. Hannah TresedderConcordia Wisconsin | 6-3, 6-1 |
| Consolation final | Virginia PurcellGrinnell College def. Emily ButlerHamline University | 8-4 |
| Consolation final | Sarah HendersonUW-Eau Claire def. Jordan DanielsonHamline University | 6-2, 6-3 |
| Consolation final | Lauren MiketinacCarroll University def. Anna GoszSt. Norbert College | 8-1 |
| Consolation final | Anna MilesEdgewood College def. Megan DavisCentral | 3-6, 6-3, 10-4 |
| Consolation final | Eliza BarterCarthage def. Gabriella FerroCornell College | 6-3, 6-0 |
| Consolation final | Elizabeth ManlickSt. Norbert College def. Maddie MuotkaHamline University | 6-1, 6-3 |
| Consolation final | Brooke VolponeUW-Eau Claire def. Jenna ZimmermanSt. Thomas | 1-0 |
| Consolation final | Casey FrommerCarthage def. Katherine RossWartburg College | 6-2, 6-2 |
| Consolation final | Judith FanGrinnell College def. Ashley MaleyCentral | 8-3 |
| Consolation round 3 | Maggie HelmsLuther def. Lilly HartmanGustavus Adolphus | 6-2, 6-1 |
| Consolation round 3 | Sadie SpahnUW-La Crosse def. Anushka JoshiGrinnell College | 6-3, 6-1 |
| Consolation round 3 | L Tamara WashingtonCoe College def. Daniela RojasSt. Scholastica | 6-3, 7-5 |
| Consolation round 3 | Sophie RobertsonCornell College def. Virginia PurcellGrinnell College | 7-5, 6-2 |
| Consolation round 3 | Sarah HendersonUW-Eau Claire def. Lauren MiketinacCarroll University | 6-2, 6-2 |
| Consolation round 3 | Anna MilesEdgewood College def. Eliza BarterCarthage | 2-6, 7-5, 11-9 |
| Consolation round 3 | Elizabeth ManlickSt. Norbert College def. Brooke VolponeUW-Eau Claire | 6-2, 6-1 |
| Consolation round 3 | Casey FrommerCarthage def. Judith FanGrinnell College | 6-1, 6-4 |
| Consolation semifinal | Maggie HelmsLuther def. Sadie SpahnUW-La Crosse | 6-4, 6-1 |
| Consolation semifinal | Sophie RobertsonCornell College def. Tamara WashingtonCoe College | 4-6, 6-4, 10-3 |
| Consolation semifinal | Sarah HendersonUW-Eau Claire def. Anna MilesEdgewood College | 6-2, 6-1 |
| Consolation semifinal | Casey FrommerCarthage def. Elizabeth ManlickSt. Norbert College | 6-2, 6-2 |
| Consolation round 5 | Maggie HelmsLuther vs. Sophie RobertsonCornell College | no result |
| Consolation round 5 | Casey FrommerCarthage def. Sarah HendersonUW-Eau Claire | 6-2, 6-3 |
