| Round of 128 | Ali HickeyGrinnell College def. Ashley MaleyCentral | 6-1, 6-2 |
| Round of 128 | Meg WittSt. Norbert College def. Tessa RahrickCollege of Saint Benedict | by default |
| Round of 128 | Cassandra BarrSaint Mary's University def. Claire BuehlerMacalester | 7-5, 6-4 |
| Round of 128 | Molly StewartEau Claire def. Julia SporerSt. Catherine | 6-2, 6-2 |
| Round of 128 | Bailey KellyConcordia Wisconsin def. Kendra StoickConcordia-M'head | 6-0, 4-6, 10-7 |
| Round of 128 | Raquel RuizCornell College def. Paige VannausdleCentral | 6-3, 6-2 |
| Round of 128 | L Paige NiermanWis.-Whitewater def. Elise HauerSt. Scholastica | 6-3, 6-0 |
| Round of 128 | Lily HamiltonGrinnell College def. Jaclyn SwiftEdgewood College | 6-2, 6-0 |
| Round of 128 | Ashley SolbergConcordia Wisconsin def. Erin McDonaldSt. Olaf College | 6-2, 6-4 |
| Round of 128 | Lauren PaulsonSt. Norbert College def. Natalie PieterickHamline | 7-5, 6-1 |
| Round of 128 | Claire ChristianLuther College def. Alaina BublitzUW-La Crosse | 6-3, 6-2 |
| Round of 128 | Laura SondaySaint Mary's University def. Brooke LapinskiNorthwestern (Minn.) | 6-3, 6-1 |
| Round of 128 | Alyssa CarewEdgewood College def. Kayla McIverCollege of Saint Benedict | 2-6, 6-1, 11-9 |
| Round of 128 | Natalie FaviaCarthage College def. EmmaGrace WalterCentral | 6-0, 6-2 |
| Round of 128 | Kendra BerryEau Claire def. Maura AndersenUW-La Crosse | 6-7 (0-5), 6-3, 10-5 |
| Round of 128 | Laurel HickeyMacalester def. Abbey NelsonCornell College | 6-1, 7-5 |
| Round of 128 | Mackenzie SchurhamerCollege of Saint Benedict def. Hannah TresedderConcordia Wisconsin | 6-3, 1-6, 10-2 |
| Round of 128 | Mackenzie NovakBethel University def. Kari GrindbergCentral | 6-2, 6-2 |
| Round of 128 | Julia BoeveSaint Mary's University def. Ali KopieckiHamline | 7-5, 6-1 |
| Round of 128 | Erin KolbowCollege of Saint Benedict def. Molly SanvikEau Claire | 6-2, 6-3 |
| Round of 128 | Abby NielsenGrinnell College def. Shanna MogdenCarroll University | 6-1, 6-2 |
| Round of 128 | L Natali SorajjaMacalester def. Daniela RojasSt. Scholastica | 7-5, 6-4 |
| Round of 128 | Sabrina BarbozaSt. Olaf College def. Sheila SullivanGustavus Adolphus | 6-4, 6-2 |
| Round of 128 | Jackie CychoszLuther College def. Sydney VerbauwhedeEdgewood College | 6-0, 6-0 |
| Round of 128 | Elizabeth CarrollConcordia Wisconsin def. Lily SteinMacalester | 6-3, 6-3 |
| Round of 128 | Jenna ForknellConcordia-M'head def. Elia HealyNebraska Wesleyan | 6-2, 6-2 |
| Round of 128 | Britta PresslerLuther College def. Marissa WilsonEdgewood College | 6-3, 6-1 |
| Round of 128 | Maegan O'HerinMacalester def. Bri DorrSt. Catherine | 6-1, 4-6, 10-6 |
| Round of 128 | Anushka JoshiGrinnell College def. Ellen StensrudCollege of Saint Benedict | 7-5, 6-2 |
| Round of 128 | Emily CooperEau Claire def. Karleigh SchillingCentral | 7-5, 6-4 |
| Round of 128 | Sadie SpahnUW-La Crosse def. Brittany GoebelWis.-Whitewater | 6-0, 6-4 |
| Round of 64 | Bridget BellissimoUW-La Crosse def. Grace KruegerCarroll University | 6-1, 6-1 |
| Round of 64 | Ali HickeyGrinnell College def. Max MellaCarthage College | 6-2, 6-2 |
| Round of 64 | Jillian WallaceWis.-Whitewater def. Meg WittSt. Norbert College | 6-1, 6-0 |
| Round of 64 | Molly StewartEau Claire def. Cassandra BarrSaint Mary's University | 6-2, 6-1 |
| Round of 64 | Haley ResnickCoe College def. Bailey KellyConcordia Wisconsin | 6-0, 6-0 |
| Round of 64 | Raquel RuizCornell College def. Bridget McKeeganNebraska Wesleyan | 7-6 (3-0), 6-3 |
| Round of 64 | Katie AneyGustavus Adolphus def. Paige NiermanWis.-Whitewater | 6-0, 6-0 |
| Round of 64 | Maggie RiermannBethel University def. Alicia PicardEdgewood College | 6-3, 6-1 |
| Round of 64 | Natalie WijesingheEau Claire def. Lily HamiltonGrinnell College | 7-5, 6-3 |
| Round of 64 | Ashley SolbergConcordia Wisconsin def. Lauren PaulsonSt. Norbert College | 6-3, 6-1 |
| Round of 64 | Ginger ValentineGustavus Adolphus def. Samanthan HindtBethel University | 7-5, 6-1 |
| Round of 64 | Claire ChristianLuther College def. Laura SondaySaint Mary's University | 6-0, 6-1 |
| Round of 64 | Arantxa MartinezDubuque, Univ. of def. Alyssa CarewEdgewood College | 6-3, 6-2 |
| Round of 64 | Amelia CoganGrinnell College def. Natalie FaviaCarthage College | 6-3, 6-3 |
| Round of 64 | Bridgid McGuireWis.-Whitewater def. Laura MartinGustavus Adolphus | 6-3, 6-1 |
| Round of 64 | Kendra BerryEau Claire def. Laurel HickeyMacalester | 6-1, 7-5 |
| Round of 64 | Julia ThomeCornell College def. Mackenzie SchurhamerCollege of Saint Benedict | 6-0, 6-0 |
| Round of 64 | Sarah HendersonEau Claire def. Casey FrommerCarthage College | 7-6 (5-0), 6-7 (0-2), 10-3 |
| Round of 64 | Jordyn LawatschSt. Norbert College def. Sabrina PalavraWis.-Whitewater | 6-3, 3-6, 10-8 |
| Round of 64 | Zoe ParkCarleton College def. Mackenzie NovakBethel University | 6-1, 6-1 |
| Round of 64 | Julia BoeveSaint Mary's University def. Erin KolbowCollege of Saint Benedict | 3-6, 6-4, 10-5 |
| Round of 64 | Hayley ClopeUW-La Crosse def. Abby NielsenGrinnell College | 3-6, 6-3, 10-6 |
| Round of 64 | Sabrina BarbozaSt. Olaf College def. Natali SorajjaMacalester | 6-3, 6-0 |
| Round of 64 | Alexandra TalyanskyWis.-Whitewater def. Jackie CychoszLuther College | 6-1, 6-1 |
| Round of 64 | Julie MartinWis.-Whitewater def. Elizabeth CarrollConcordia Wisconsin | 6-0, 6-1 |
| Round of 64 | Briana HartmannGustavus Adolphus def. Gabriella FerroCornell College | 6-0, 6-0 |
| Round of 64 | Angelica EmmanouilCarthage College def. Jenna ForknellConcordia-M'head | 6-1, 6-4 |
| Round of 64 | Milica CvrkotaGrinnell College def. Britta PresslerLuther College | 6-4, 6-1 |
| Round of 64 | Anushka JoshiGrinnell College def. Maegan O'HerinMacalester | 2-6, 7-6 (3-0), 10-3 |
| Round of 64 | Lauren FrenchCoe College def. Emily CooperEau Claire | 6-2, 6-0 |
| Round of 64 | Mattie KidderBethel University def. Sadie SpahnUW-La Crosse | 7-5, 6-4 |
| Round of 64 | Danielle VasilievCarleton College def. Hannah BorghGustavus Adolphus | 6-2, 6-1 |
| Round of 32 | Bridget BellissimoUW-La Crosse def. Ali HickeyGrinnell College | 6-3, 6-4 |
| Round of 32 | Jillian WallaceWis.-Whitewater def. Molly StewartEau Claire | 6-1, 6-2 |
| Round of 32 | Haley ResnickCoe College def. Raquel RuizCornell College | 6-2, 6-0 |
| Round of 32 | Maggie RiermannBethel University def. Katie AneyGustavus Adolphus | 4-6, 6-2, 13-11 |
| Round of 32 | Natalie WijesingheEau Claire def. Ashley SolbergConcordia Wisconsin | 6-3, 6-3 |
| Round of 32 | Ginger ValentineGustavus Adolphus def. Laura SondaySaint Mary's University | 7-5, 6-0 |
| Round of 32 | Arantxa MartinezDubuque, Univ. of def. Amelia CoganGrinnell College | 0-6, 6-4, 10-8 |
| Round of 32 | Bridgid McGuireWis.-Whitewater def. Kendra BerryEau Claire | 6-0, 6-2 |
| Round of 32 | Sarah HendersonEau Claire def. Julia ThomeCornell College | 3-6, 6-4, 10-6 |
| Round of 32 | Zoe ParkCarleton College def. Jordyn LawatschSt. Norbert College | 6-1, 6-2 |
| Round of 32 | Hayley ClopeUW-La Crosse def. Julia BoeveSaint Mary's University | 6-2, 6-0 |
| Round of 32 | Alexandra TalyanskyWis.-Whitewater def. Sabrina BarbozaSt. Olaf College | 6-1, 6-0 |
| Round of 32 | Briana HartmannGustavus Adolphus def. Julie MartinWis.-Whitewater | 6-7 (0-3), 6-4, 10-8 |
| Round of 32 | Milica CvrkotaGrinnell College def. Angelica EmmanouilCarthage College | 6-4, 6-1 |
| Round of 32 | Lauren FrenchCoe College def. Anushka JoshiGrinnell College | 6-0, 6-1 |
| Round of 32 | Danielle VasilievCarleton College def. Mattie KidderBethel University | 6-4, 6-1 |
| Round of 16 | Jillian WallaceWis.-Whitewater def. Bridget BellissimoUW-La Crosse | 6-4, 6-3 |
| Round of 16 | Maggie RiermannBethel University def. Haley ResnickCoe College | 4-6, 6-4, 6-3 |
| Round of 16 | Natalie WijesingheEau Claire def. Ginger ValentineGustavus Adolphus | 6-4, 6-3 |
| Round of 16 | Bridgid McGuireWis.-Whitewater def. Arantxa MartinezDubuque, Univ. of | 6-1, 7-6 (4-0) |
| Round of 16 | Zoe ParkCarleton College def. Sarah HendersonEau Claire | 7-6 (4-0), 6-1 |
| Round of 16 | Alexandra TalyanskyWis.-Whitewater def. Hayley ClopeUW-La Crosse | 6-1, 6-0 |
| Round of 16 | Briana HartmannGustavus Adolphus def. Milica CvrkotaGrinnell College | 4-6, 6-1, 6-1 |
| Round of 16 | Lauren FrenchCoe College def. Danielle VasilievCarleton College | 6-2, 6-2 |
| Quarterfinal | Jillian WallaceWis.-Whitewater def. Maggie RiermannBethel University | 6-4, 6-0 |
| Quarterfinal | Natalie WijesingheEau Claire def. Bridgid McGuireWis.-Whitewater | 6-3, 7-6 (6-0) |
| Quarterfinal | Alexandra TalyanskyWis.-Whitewater def. Zoe ParkCarleton College | 6-1, 6-0 |
| Consolation round of 64 | Claire BuehlerMacalester def. Julia SporerSt. Catherine | 6-4, 6-1 |
| Consolation round of 64 | Bridget McKeeganNebraska Wesleyan def. Paige VannausdleCentral | 6-4, 6-2 |
| Consolation round of 64 | Erin McDonaldSt. Olaf College def. Natalie PieterickHamline | 6-2, 6-1 |
| Consolation round of 64 | Brooke LapinskiNorthwestern (Minn.) def. Alaina BublitzUW-La Crosse | 6-1, 6-1 |
| Consolation round of 64 | Ali KopieckiHamline def. Molly SanvikEau Claire | 6-2, 2-6, 10-5 |
| Consolation round of 64 | L Sheila SullivanGustavus Adolphus def. Daniela RojasSt. Scholastica | 7-5, 6-1 |
| Consolation round of 64 | Bri DorrSt. Catherine def. Ellen StensrudCollege of Saint Benedict | 4-6, 6-2, 11-9 |
| Consolation round of 32 | Grace KruegerCarroll University def. Ashley MaleyCentral | 6-0, 6-0 |
| Consolation round of 32 | Bridget McKeeganNebraska Wesleyan def. Kendra StoickConcordia-M'head | 6-4, 6-3 |
| Consolation round of 32 | L Alicia PicardEdgewood College def. Elise HauerSt. Scholastica | 6-3, 7-6 (5-0) |
| Consolation round of 32 | Erin McDonaldSt. Olaf College def. Jaclyn SwiftEdgewood College | 6-0, 6-3 |
| Consolation round of 32 | Brooke LapinskiNorthwestern (Minn.) def. Alishia WeidmeierSt. Catherine | 6-4, 6-3 |
| Consolation round of 32 | Kayla McIverCollege of Saint Benedict def. EmmaGrace WalterCentral | 7-6 (4-0), 6-1 |
| Consolation round of 32 | Maura AndersenUW-La Crosse def. Laura MartinGustavus Adolphus | 6-3, 6-3 |
| Consolation round of 32 | Sabrina PalavraWis.-Whitewater def. Kari GrindbergCentral | 6-4, 6-2 |
| Consolation round of 32 | Shanna MogdenCarroll University def. Ali KopieckiHamline | 2-6, 6-3, 10-4 |
| Consolation round of 32 | Sydney VerbauwhedeEdgewood College def. Sheila SullivanGustavus Adolphus | 6-0, 4-6, 10-8 |
| Consolation round of 32 | Lily SteinMacalester def. Gabriella FerroCornell College | by default |
| Consolation round of 32 | Marissa WilsonEdgewood College def. Elia HealyNebraska Wesleyan | 6-3, 6-0 |
| Consolation round of 32 | Karleigh SchillingCentral def. Bri DorrSt. Catherine | 6-2, 6-4 |
| Consolation round of 32 | Brittany GoebelWis.-Whitewater def. Hannah BorghGustavus Adolphus | 6-0, 5-7, 10-7 |
| Consolation round of 16 | Grace KruegerCarroll University def. Claire BuehlerMacalester | 4-6, 6-2, 10-3 |
| Consolation round of 16 | Alicia PicardEdgewood College def. Bridget McKeeganNebraska Wesleyan | 5-7, 6-4, 12-10 |
| Consolation round of 16 | Erin McDonaldSt. Olaf College def. Brooke LapinskiNorthwestern (Minn.) | 6-2, 3-6, 10-2 |
| Consolation round of 16 | Maura AndersenUW-La Crosse def. Kayla McIverCollege of Saint Benedict | 6-3, 6-4 |
| Consolation round of 16 | Sabrina PalavraWis.-Whitewater def. Hannah TresedderConcordia Wisconsin | 6-3, 6-2 |
| Consolation round of 16 | Shanna MogdenCarroll University def. Sydney VerbauwhedeEdgewood College | 5-7, 7-5, 10-6 |
| Consolation round of 16 | Marissa WilsonEdgewood College def. Lily SteinMacalester | by default |
| Consolation round of 16 | Brittany GoebelWis.-Whitewater def. Karleigh SchillingCentral | 6-3, 6-3 |
| Consolation quarterfinal | Grace KruegerCarroll University def. Alicia PicardEdgewood College | 6-3, 6-1 |
| Consolation quarterfinal | Maura AndersenUW-La Crosse def. Erin McDonaldSt. Olaf College | 6-0, 6-2 |
| Consolation quarterfinal | Sabrina PalavraWis.-Whitewater def. Shanna MogdenCarroll University | 6-4, 6-7 (0-6), 10-7 |
| Consolation quarterfinal | Brittany GoebelWis.-Whitewater def. Marissa WilsonEdgewood College | 6-0, 6-2 |
| Consolation round of 64 | Ashley MaleyCentral def. Max MellaCarthage College | by default |
| Quarterfinal | Briana HartmannGustavus Adolphus def. Lauren FrenchCoe College | 6-3, 6-4 |
| Semifinal | Jillian WallaceWis.-Whitewater def. Natalie WijesingheEau Claire | 6-3, 7-5 |
| Semifinal | Briana HartmannGustavus Adolphus def. Alexandra TalyanskyWis.-Whitewater | 6-2, 6-3 |
| Final | Briana HartmannGustavus Adolphus def. Jillian WallaceWis.-Whitewater | 6-1, 6-1 |
