| Round of 64 | Kathryn SchmidtCarleton College def. Sydney DivelbissUW-Whitewater | 6-4, 6-4 |
| Round of 64 | Jackie PedersenCoe College def. Lola White-BaerLuther College | 6-0, 6-2 |
| Round of 64 | Taylor HeltneUW-Eau Claire def. Annie MahoneyGustavus Adolphus College | 6-2, 6-2 |
| Round of 64 | Christina SchauppLawrence University def. Michaela BurroughsSt. Catherine University | 6-2, 7-6 |
| Round of 64 | Hollis McPeekCarthage def. Bailey EdwardsSaint Mary's University | 6-4, 7-5 |
| Round of 64 | Sadhana AthreyaGrinnell College def. Ann YahbroughHamline University | 6-2, 6-0 |
| Round of 64 | Andrea RognlienConcordia College def. Emily RoedigerGustavus Adolphus College | 7-5, 6-4 |
| Round of 64 | Olivia HartwickUW-La Crosse def. Kristen BooneEdgewood College | 6-0, 6-1 |
| Round of 64 | L Kalla SchaeferUW-Whitewater def. Amanda HiattCollege of St. Scholastica | 6-2, 6-0 |
| Round of 64 | Elise AllenLuther College def. Kristina NaidiczCarthage | 6-4, 7-6 (7-5) |
| Round of 64 | Tai LuceroCoe College def. Constanza AlarconGrinnell College | 6-0, 6-1 |
| Round of 64 | Megan BornGustavus Adolphus College def. Jenny MaruyamaUW-La Crosse | 6-3, 7-6 (7-5) |
| Round of 64 | Alex EricksonGustavus Adolphus College def. Megan KastenUW-Whitewater | 6-0, 6-2 |
| Round of 64 | Toby CainGrinnell College def. Hannah GeoffreyLawrence University | 6-1, 6-2 |
| Round of 64 | Caitlin HarperSt. Olaf College def. Abby WerwieUW-Stout | 6-2, 6-1 |
| Round of 64 | Cassandra ChalhoubLuther College def. Mackenzie WeberSt. Catherine University | 6-2, 6-2 |
| Round of 64 | Jennifer RoeschLawrence University def. Erin HenricksonCarthage | 6-0, 6-3 |
| Round of 64 | McKennah EdwardsSaint Mary's University def. Katie GillmanUW-Eau Claire | 6-3, 6-4 |
| Round of 64 | Hanna FeldmanGrinnell College def. Lauren AguilarUW-Whitewater | 3-6, 6-3, 6-2 |
| Round of 64 | Anne LombardiCarleton College def. Nicole KrierHamline University | 6-1, 6-1 |
| Round of 64 | Laurel KrebsbachGustavus Adolphus College def. Laura OlsonEdgewood College | 6-0, 6-0 |
| Round of 64 | Annie SmithUW-Whitewater def. Andrea VivianUW-La Crosse | 6-2, 6-1 |
| Round of 64 | W Hillary BungardenCollege of St. Scholastica def. Gayatri MalhotraLawrence University | 4-6, 6-1, 6-4 |
| Round of 64 | Michelle SchupbachCoe College def. Gretchen BachmeierUW-Eau Claire | 6-2, 6-2 |
| Round of 64 | Alexandra BaylissUW-Whitewater def. Bari ReynoldsCarthage | 7-5, 6-3 |
| Round of 64 | Erin GudulCarleton College def. Clothilde ThirouinGrinnell College | 6-0, 6-0 |
| Round of 64 | Sara OhlmanCoe College def. Carissa WallerichHamline University | 6-1, 6-2 |
| Round of 64 | Elizabeth LarsonLawrence University def. Maria KovalSt. Catherine University | 6-4, 6-1 |
| Round of 64 | Shirlene LukGrinnell College def. Jackie VitaleUW-Whitewater | 6-4, 7-6 (6-4) |
| Round of 64 | Cami CzechLuther College def. Christine MullerSt. Olaf College | 6-3, 6-3 |
| Round of 64 | Mara WeinerUW-La Crosse def. Angie LorenzenSt. Catherine University | 6-1, 6-0 |
| Round of 64 | Megan GaardGustavus Adolphus College def. Taylor StorbakkenEdgewood College | 6-0, 6-0 |
| Round of 32 | Kathryn SchmidtCarleton College def. Jackie PedersenCoe College | 6-1, 6-0 |
| Round of 32 | Taylor HeltneUW-Eau Claire def. Christina SchauppLawrence University | 6-1, 7-6 (6-4) |
| Round of 32 | Sadhana AthreyaGrinnell College def. Hollis McPeekCarthage | 7-5, 6-4 |
| Round of 32 | Andrea RognlienConcordia College def. Olivia HartwickUW-La Crosse | 7-5, 6-0 |
| Round of 32 | Kalla SchaeferUW-Whitewater def. Elise AllenLuther College | 6-1, 6-1 |
| Round of 32 | Tai LuceroCoe College def. Megan BornGustavus Adolphus College | 6-0, 6-0 |
| Round of 32 | Alex EricksonGustavus Adolphus College def. Toby CainGrinnell College | 6-3, 6-1 |
| Round of 32 | Cassandra ChalhoubLuther College def. Caitlin HarperSt. Olaf College | 1-6, 6-4, 6-1 |
| Round of 32 | McKennah EdwardsSaint Mary's University def. Jennifer RoeschLawrence University | 6-1, 6-0 |
| Round of 32 | Anne LombardiCarleton College def. Hanna FeldmanGrinnell College | 6-3, 7-5 |
| Round of 32 | Laurel KrebsbachGustavus Adolphus College def. Annie SmithUW-Whitewater | 6-2, 6-1 |
| Round of 32 | L Michelle SchupbachCoe College def. Hillary BungardenCollege of St. Scholastica | 6-2, 6-0 |
| Round of 32 | Alexandra BaylissUW-Whitewater def. Erin GudulCarleton College | 6-0, 6-2 |
| Round of 32 | Elizabeth LarsonLawrence University def. Sara OhlmanCoe College | 6-0, 6-1 |
| Round of 32 | Cami CzechLuther College def. Shirlene LukGrinnell College | 6-4, 6-0 |
| Round of 32 | Megan GaardGustavus Adolphus College def. Mara WeinerUW-La Crosse | 6-3, 6-0 |
| Round of 16 | Kathryn SchmidtCarleton College def. Taylor HeltneUW-Eau Claire | 6-4, 6-3 |
| Round of 16 | Andrea RognlienConcordia College def. Sadhana AthreyaGrinnell College | 7-5, 6-4 |
| Round of 16 | Kalla SchaeferUW-Whitewater def. Tai LuceroCoe College | 4-6, 6-3, 6-2 |
| Round of 16 | Alex EricksonGustavus Adolphus College def. Cassandra ChalhoubLuther College | 6-3, 6-3 |
| Round of 16 | McKennah EdwardsSaint Mary's University def. Anne LombardiCarleton College | 7-6 (6-2), 6-3 |
| Round of 16 | Laurel KrebsbachGustavus Adolphus College def. Michelle SchupbachCoe College | 6-3, 6-2 |
| Round of 16 | Alexandra BaylissUW-Whitewater def. Elizabeth LarsonLawrence University | 7-5, 6-3 |
| Round of 16 | Cami CzechLuther College def. Megan GaardGustavus Adolphus College | 6-0, 6-3 |
| Quarterfinal | Kathryn SchmidtCarleton College def. Andrea RognlienConcordia College | 6-2, 6-1 |
| Quarterfinal | Kalla SchaeferUW-Whitewater def. Alex EricksonGustavus Adolphus College | 6-3, 6-2 |
| Quarterfinal | McKennah EdwardsSaint Mary's University def. Laurel KrebsbachGustavus Adolphus College | 6-2, 6-3 |
| Quarterfinal | Alexandra BaylissUW-Whitewater def. Cami CzechLuther College | 7-5, 6-3 |
| Semifinal | Kalla SchaeferUW-Whitewater def. Kathryn SchmidtCarleton College | 6-2, 5-7, 6-3 |
| Semifinal | McKennah EdwardsSaint Mary's University def. Alexandra BaylissUW-Whitewater | 6-2, 6-2 |
| Final | Kalla SchaeferUW-Whitewater def. McKennah EdwardsSaint Mary's University | 6-2, 6-2 |
