| Round of 128 | Emily CooperUW-Eau Claire def. Stephanie BushCoe College | 6-3, 6-3 |
| Round of 128 | Hannah BorghGustavus Adolphus College def. Allie MillerWis.-Whitewater | 3-6, 7-5, 7-5 |
| Round of 128 | Britta PresslerLuther College def. Emma GaydosGrinnell College | 6-7, 6-2, 6-0 |
| Round of 128 | Ricki BudewitzConcordia Wisconsin def. Samantha KoppaUW-Oshkosh | 6-4, 6-2 |
| Round of 128 | Sydney VerbauwhedeEdgewood College def. Allie MillerWis.-Whitewater | 6-4, 6-3 |
| Round of 128 | W Elizabeth FinkSt. Scholastica def. Karleigh SchillingCentral | 3-6, 6-2, 6-2 |
| Round of 128 | Abbey NelsonCornell College def. Ellie BergstrandSt. Olaf College | 4-6, 7-6, 6-4 |
| Round of 128 | Shanna MogdenCarroll def. Emily KingSimpson | 7-5, 6-4 |
| Round of 128 | Natali SorajjaMacalester def. Nia PhamCarthage College | 3-6, 6-1, 6-1 |
| Round of 128 | Kaitlyn KalinkaUW-La Crosse def. Olivia KraftWis.-Whitewater | 1-6, 6-4, 7-5 |
| Round of 128 | Sydney RottBethel University def. Cami GriciusCornell College | 6-7, 6-2, 6-2 |
| Round of 128 | Jill WeinzettelBethel University def. Hannah PetersUW-Oshkosh | 6-1, 6-1 |
| Round of 128 | Kellis BrandtSt. Olaf College def. Colleen LoisSaint Mary's University | 6-1, 7-5 |
| Round of 128 | Sabrina PalavraWis.-Whitewater def. Isabel GreenGrinnell College | 7-5, 6-4 |
| Round of 128 | Bri DorrSt. Catherine University def. Natalie PieterickHamline | 6-0, 6-2 |
| Round of 128 | Shelby CookLuther College def. Lily HamiltonGrinnell College | 6-1, 6-3 |
| Round of 128 | Alyssa BoyerUW-La Crosse def. Taylor KopferBethel University | 6-1, 6-2 |
| Round of 128 | Cassandra BarrSaint Mary's University def. Eliza SundermannCoe College | 2-6, 6-2, 6-1 |
| Round of 128 | Meg WittSt. Norbert College def. Emma BakerMacalester | 6-4, 6-7, 6-3 |
| Round of 128 | Nikki PasketwitzNorthwestern (Minn.) def. Raquel RuizCornell College | 6-2, 6-2 |
| Round of 128 | Madeline SlickerWis.-Whitewater def. Alyssa CarewEdgewood College | 6-4, 4-6, 7-5 |
| Round of 128 | Jenna ForknellConcordia-M'head def. Kayla ReuscheSimpson | 6-3, 6-2 |
| Round of 128 | Sabrina BarbozaSt. Olaf College def. Maura AndersenUW-La Crosse | 6-3, 1-6, 6-1 |
| Round of 128 | Sheila SullivanGustavus Adolphus College def. Michelle SpicerUW-Oshkosh | 6-0, 6-1 |
| Round of 128 | Elise HaWis.-Whitewater def. Tessa RuggHamline | 6-0, 6-1 |
| Round of 128 | Devon BourgetLuther College def. Alisha WiedmeirSt. Catherine University | 7-6, 6-4 |
| Round of 128 | Natalie FaviaCarthage College def. Jenna StewartCentral | 7-6, 6-2 |
| Round of 128 | Sarah BenoySt. Thomas def. Bailey KellyConcordia Wisconsin | 6-1, 6-2 |
| Round of 128 | Laurel HickeyMacalester def. Julia BoeveSaint Mary's University | 1-6, 6-4, 6-2 |
| Round of 128 | Jessica MinkovWis.-Whitewater def. Isabella HoppenjansCoe College | 6-0, 6-3 |
| Round of 128 | L Elizabeth YimCarleton College def. Elise HauerSt. Scholastica | 6-4, 6-3 |
| Round of 64 | Gabi KitchellWis.-Whitewater def. Emily CooperUW-Eau Claire | 6-1, 6-1 |
| Round of 64 | Laura SondaySaint Mary's University def. Hannah BorghGustavus Adolphus College | 3-6, 6-0, 7-5 |
| Round of 64 | Angelica EmmanouilCarthage College def. Britta PresslerLuther College | 6-1, 6-4 |
| Round of 64 | Maggie RiermannBethel University def. Sydney VerbauwhedeEdgewood College | 6-3, 6-0 |
| Round of 64 | L Yelena HallmanCarleton College def. Elizabeth FinkSt. Scholastica | 6-3, 6-2 |
| Round of 64 | Lilly HartmanGustavus Adolphus College def. Abbey NelsonCornell College | 6-2, 6-3 |
| Round of 64 | Sadie SpahnUW-La Crosse def. Shanna MogdenCarroll | 6-0, 6-1 |
| Round of 64 | Ginger ValentineGustavus Adolphus College def. Natali SorajjaMacalester | 6-2, 6-1 |
| Round of 64 | Ashley SolbergConcordia Wisconsin def. Kaitlyn KalinkaUW-La Crosse | 6-0, 7-5 |
| Round of 64 | Lauren FrenchCoe College def. Sydney RottBethel University | 6-2, 6-3 |
| Round of 64 | Jill WeinzettelBethel University def. Anna LearySt. Thomas | 7-6, 6-1 |
| Round of 64 | Kellis BrandtSt. Olaf College def. Jeanny ZhangCarleton College | by default |
| Round of 64 | Sabrina PalavraWis.-Whitewater def. Hayley ClopeUW-La Crosse | 6-4, 6-4 |
| Round of 64 | Natalie WijesingheUW-Eau Claire def. Bri DorrSt. Catherine University | 6-0, 6-2 |
| Round of 64 | Anna StutzGustavus Adolphus College def. Hannah FoxWartburg | 6-0, 6-1 |
| Round of 64 | Shelby CookLuther College def. Ally ThorsenGustavus Adolphus College | 6-1, 6-3 |
| Round of 64 | Julie MartinWis.-Whitewater def. Alyssa BoyerUW-La Crosse | 6-4, 6-0 |
| Round of 64 | Cassandra BarrSaint Mary's University def. Alyssa LEfflerUW-Oshkosh | 6-2, 6-3 |
| Round of 64 | Ali KopieckiHamline def. Meg WittSt. Norbert College | 6-1, 6-0 |
| Round of 64 | Sam RamseyCarthage College def. Nikki PasketwitzNorthwestern (Minn.) | 0-6, 7-5, 6-4 |
| Round of 64 | Simona PotockovaGustavus Adolphus College def. Madeline SlickerWis.-Whitewater | 6-2, 6-4 |
| Round of 64 | Mackenzie NovakBethel University def. Jenna ForknellConcordia-M'head | 6-1, 6-1 |
| Round of 64 | Sonya RomanenkoCarleton College def. Sabrina BarbozaSt. Olaf College | 6-2, 6-3 |
| Round of 64 | Molly StewartUW-Eau Claire def. Sheila SullivanGustavus Adolphus College | 7-5, 6-1 |
| Round of 64 | Grace RiermannBethel University def. Elise HaWis.-Whitewater | 6-2, 6-1 |
| Round of 64 | Devon BourgetLuther College def. Ashley RottinghausWartburg | 6-4, 6-1 |
| Round of 64 | Abby NielsenGrinnell College def. Natalie FaviaCarthage College | 7-6, 6-2 |
| Round of 64 | Sarah BenoySt. Thomas def. Alicia PicardEdgewood College | 6-2, 6-0 |
| Round of 64 | Kimberly SteinertUW-La Crosse def. Laurel HickeyMacalester | 6-0, 7-6 |
| Round of 64 | Grace KruegerCarroll def. Jessica MinkovWis.-Whitewater | 6-1, 6-3 |
| Round of 64 | Bri HartmannGustavus Adolphus College def. Elizabeth YimCarleton College | 6-0, 6-1 |
| Round of 32 | Gabi KitchellWis.-Whitewater def. Laura SondaySaint Mary's University | 6-0, 6-2 |
| Round of 32 | Brooke HapukuSt. Thomas def. Angelica EmmanouilCarthage College | 6-2, 6-0 |
| Round of 32 | Maggie RiermannBethel University def. Yelena HallmanCarleton College | 6-1, 6-2 |
| Round of 32 | Lilly HartmanGustavus Adolphus College def. Sadie SpahnUW-La Crosse | 6-3, 6-0 |
| Round of 32 | Ginger ValentineGustavus Adolphus College def. Ashley SolbergConcordia Wisconsin | 6-0, 6-0 |
| Round of 32 | Jill WeinzettelBethel University def. Lauren FrenchCoe College | 3-6, 6-4, 6-2 |
| Round of 32 | Sabrina PalavraWis.-Whitewater def. Kellis BrandtSt. Olaf College | 6-2, 7-5 |
| Round of 32 | Natalie WijesingheUW-Eau Claire def. Anna StutzGustavus Adolphus College | 6-3, 6-2 |
| Round of 32 | Julie MartinWis.-Whitewater def. Ally ThorsenGustavus Adolphus College | 6-1, 6-0 |
| Round of 32 | Ali KopieckiHamline def. Cassandra BarrSaint Mary's University | 6-1, 6-0 |
| Round of 32 | Simona PotockovaGustavus Adolphus College def. Sam RamseyCarthage College | 2-6, 6-3, 6-2 |
| Round of 32 | Sonya RomanenkoCarleton College def. Mackenzie NovakBethel University | 6-2, 7-6 |
| Round of 32 | Grace RiermannBethel University def. Molly StewartUW-Eau Claire | 6-1, 6-1 |
| Round of 32 | Abby NielsenGrinnell College def. Devon BourgetLuther College | 6-1, 6-2 |
| Round of 32 | Kimberly SteinertUW-La Crosse def. Sarah BenoySt. Thomas | 7-5, 6-2 |
| Round of 32 | Bri HartmannGustavus Adolphus College def. Grace KruegerCarroll | 6-2, 6-2 |
| Round of 16 | Gabi KitchellWis.-Whitewater def. Brooke HapukuSt. Thomas | 6-0, 6-0 |
| Round of 16 | Maggie RiermannBethel University def. Lilly HartmanGustavus Adolphus College | 6-0, 6-4 |
| Round of 16 | Ginger ValentineGustavus Adolphus College def. Jill WeinzettelBethel University | 6-0, 6-3 |
| Round of 16 | Natalie WijesingheUW-Eau Claire def. Sabrina PalavraWis.-Whitewater | 6-3, 6-2 |
| Round of 16 | Ali KopieckiHamline def. Julie MartinWis.-Whitewater | 6-3, 6-7, 6-3 |
| Round of 16 | Sonya RomanenkoCarleton College def. Simona PotockovaGustavus Adolphus College | 6-2, 6-1 |
| Round of 16 | Grace RiermannBethel University def. Abby NielsenGrinnell College | 6-3, 6-1 |
| Round of 16 | Bri HartmannGustavus Adolphus College def. Kimberly SteinertUW-La Crosse | 6-2, 6-2 |
| Quarterfinal | Gabi KitchellWis.-Whitewater def. Maggie RiermannBethel University | 6-3, 6-3 |
| Quarterfinal | Ginger ValentineGustavus Adolphus College def. Natalie WijesingheUW-Eau Claire | 6-2, 7-5 |
| Quarterfinal | Ali KopieckiHamline def. Sonya RomanenkoCarleton College | 6-4, 6-1 |
| Quarterfinal | Grace RiermannBethel University def. Bri HartmannGustavus Adolphus College | 6-3, 6-4 |
| Semifinal | Ginger ValentineGustavus Adolphus College def. Gabi KitchellWis.-Whitewater | 6-4, 2-6, 6-3 |
| Semifinal | Ali KopieckiHamline def. Grace RiermannBethel University | 7-6, 7-5 |
| Final | Ginger ValentineGustavus Adolphus College def. Ali KopieckiHamline | 6-0, 6-4 |
| Consolation round of 64 | Anna LearySt. Thomas def. Hannah PetersUW-Oshkosh | 6-1, 6-0 |
| Consolation round of 64 | Hayley ClopeUW-La Crosse def. Isabel GreenGrinnell College | 3-6, 6-0, 10-8 |
| Consolation round of 64 | Alyssa LEfflerUW-Oshkosh def. Eliza SundermannCoe College | 6-1, 6-1 |
| Consolation round of 64 | Alisha WiedmeirSt. Catherine University def. Ashley RottinghausWartburg | by default |
| Consolation round of 64 | Alicia PicardEdgewood College def. Bailey KellyConcordia Wisconsin | 7-6, 2-6, 11-9 |
| Consolation round of 32 | Stephanie BushCoe College def. Della BrownMacalester | by default |
| Consolation round of 32 | Emma GaydosGrinnell College def. Samantha KoppaUW-Oshkosh | 3-6, 6-3, 10-5 |
| Consolation round of 32 | Allie MillerWis.-Whitewater def. Karleigh SchillingCentral | 6-4, 2-6, 10-7 |
| Consolation round of 32 | Emily KingSimpson def. Ellie BergstrandSt. Olaf College | 6-2, 7-6 (1-0) |
| Consolation round of 32 | Olivia KraftWis.-Whitewater def. Nia PhamCarthage College | 3-6, 6-4, 10-7 |
| Consolation round of 32 | Anna LearySt. Thomas def. Cami GriciusCornell College | 6-3, 6-2 |
| Consolation round of 32 | Hayley ClopeUW-La Crosse def. Colleen LoisSaint Mary's University | 7-5, 6-2 |
| Consolation round of 32 | Hannah FoxWartburg def. Natalie PieterickHamline | 6-0, 6-2 |
| Consolation round of 32 | Taylor KopferBethel University def. Lily HamiltonGrinnell College | 6-3, 6-2 |
| Consolation round of 32 | Alyssa LEfflerUW-Oshkosh def. Emma BakerMacalester | 6-1, 6-1 |
| Consolation round of 32 | Alyssa CarewEdgewood College def. Raquel RuizCornell College | 7-6 (4-0), 6-2 |
| Consolation round of 32 | Maura AndersenUW-La Crosse def. Kayla ReuscheSimpson | 6-2, 6-3 |
| Consolation round of 32 | Michelle SpicerUW-Oshkosh def. Tessa RuggHamline | 6-3, 6-3 |
| Consolation round of 32 | Alisha WiedmeirSt. Catherine University def. Jenna StewartCentral | 6-2, 7-6 (1-0) |
| Consolation round of 32 | Julia BoeveSaint Mary's University def. Alicia PicardEdgewood College | 6-0, 6-1 |
| Consolation round of 32 | L Isabella HoppenjansCoe College def. Elise HauerSt. Scholastica | by default |
| Consolation round of 16 | Emma GaydosGrinnell College def. Stephanie BushCoe College | 6-0, 3-6, 10-5 |
| Consolation round of 16 | Allie MillerWis.-Whitewater def. Emily KingSimpson | 7-6 (3-0), 6-3 |
| Consolation round of 16 | Anna LearySt. Thomas def. Olivia KraftWis.-Whitewater | 7-6 (5-0), 6-2 |
| Consolation round of 16 | Hayley ClopeUW-La Crosse def. Hannah FoxWartburg | 6-0, 6-0 |
| Consolation round of 16 | Alyssa LEfflerUW-Oshkosh def. Taylor KopferBethel University | 6-4, 6-3 |
| Consolation round of 16 | Maura AndersenUW-La Crosse def. Alyssa CarewEdgewood College | 6-4, 6-0 |
| Consolation round of 16 | Alisha WiedmeirSt. Catherine University def. Michelle SpicerUW-Oshkosh | 6-0, 7-5 |
| Consolation round of 16 | Isabella HoppenjansCoe College def. Julia BoeveSaint Mary's University | 6-2, 4-6, 10-7 |
