| Round of 128 | Lily PerrinGrinnell College def. Luana Da SilvaCarthage College | 6-0, 6-0 |
| Round of 128 | Cecile FuchsEdgewood College def. Mallory MoenConcordia Wisconsin | 6-0, 6-2 |
| Round of 128 | Emily NormanGustavus Adolphus def. Morgan GrafUW-La Crosse | 6-2, 4-6, 1-0 (12-10) |
| Round of 128 | Raquel EggeConcordia Moorhead def. Sarah SchirmCentral | 6-2, 6-0 |
| Round of 128 | Rachel LindrudGustavus Adolphus def. Sydney BellCarleton College | 7-6 (7-3), 6-2 |
| Round of 128 | Sofia SackettLuther College def. Lindsey HendersonWis.-Eau Claire | 6-4, 6-3 |
| Round of 128 | Maya CallawayUW-Superior def. Elena BourgetWis.-Eau Claire | 6-3, 6-0 |
| Round of 128 | W Julia MaierSt. Scholastica def. Courtney DayWis.-Whitewater | 6-2, 6-4 |
| Round of 128 | Sydney RottBethel (MN) def. Zoie BeckerSaint Mary's University | 6-2, 6-0 |
| Round of 128 | Renata HernandezGustavus Adolphus def. Katie LarsonCentral | 6-2, 6-1 |
| Round of 128 | Meadow LaDukeWartburg def. Elsa JolySt. Catherine | 6-7 (4-7), 6-3, 1-0 (10-3) |
| Round of 128 | Sam RamseyCarthage College def. Kennedy MorganSaint Mary's University | 6-2, 6-2 |
| Round of 128 | Ashley LaBelleCarroll (WI) def. Alexa BrooksWis.-Eau Claire | 7-5, 6-2 |
| Round of 128 | Cheyanne PhillipsConcordia Wisconsin def. Molly RuethWis.-Whitewater | 6-2, 7-6 (7-2) |
| Round of 128 | Samantha FuchsWis.-Eau Claire def. Elise BierbaumBethel (MN) | 6-3, 6-2 |
| Round of 128 | Kaitlyn KalinkaUW-La Crosse def. Meg UlrichMacalester | 6-3, 7-5 |
| Round of 128 | Halle RosentreterGustavus Adolphus def. Amanye ReynoldsMacalester | 6-2, 3-6, 1-0 (10-5) |
| Round of 128 | Madalyn BigelowWis.-Whitewater def. Ally VerbauwhedeEdgewood College | 6-4, 6-4 |
| Round of 128 | Alli LauxGustavus Adolphus def. Ricki BudewitzConcordia Wisconsin | 7-5, 7-5 |
| Round of 128 | Jenna ForknellConcordia Moorhead def. Zenia BarrettoWis.-Whitewater | 5-7, 6-3, 1-0 (10-8) |
| Round of 128 | Elaine FrantaWis.-Eau Claire def. Barbara CovekUW Stevens Point | 6-2, 6-2 |
| Round of 128 | Utsah KalraGrinnell College def. Ana OlimpioLuther College | 7-6 (8-6), 6-2 |
| Round of 128 | Hayley TrebilGustavus Adolphus def. Emma TholeBethel (MN) | 6-7 (5-7), 6-4, 1-0 (10-8) |
| Round of 128 | Sydney PreslerWis.-Eau Claire def. Hannah RiegleUW-La Crosse | 6-3, 4-6, 1-0 (10-7) |
| Round of 128 | Megan KarrowGustavus Adolphus def. Bronwyn MetcalfCentral | 4-6, 6-1, 1-0 (10-6) |
| Round of 128 | Ashley SamutaConcordia Wisconsin def. Kellie HaWis.-Whitewater | 6-4, 5-7, 1-0 (10-5) |
| Round of 128 | Sophia de los ReyesGrinnell College def. Susi HuUW-La Crosse | 6-2, 6-0 |
| Round of 128 | L Natalia SmithLuther College def. Madilyn JankilaSt. Scholastica | 6-1, 6-2 |
| Round of 128 | Sofia WalkerSt. Catherine def. Rylee SchurmanBethel (MN) | 6-2, 6-3 |
| Round of 128 | Emily KingSimpson def. Kaytlyn SyversonUW-Superior | 6-2, 6-0 |
| Round of 128 | Maggie FryeSaint Mary's University def. Alyssa CarewEdgewood College | 6-3, 7-6 (7-4) |
| Round of 64 | Grace RiermannBethel (MN) def. Lily PerrinGrinnell College | 6-4, 6-0 |
| Round of 64 | Cecile FuchsEdgewood College def. Sydney DouglasGustavus Adolphus | 6-1, 6-1 |
| Round of 64 | Ioanna GiannakouGrinnell College def. Emily NormanGustavus Adolphus | 7-6 (7-3), 3-6, 1-0 (11-9) |
| Round of 64 | Raquel EggeConcordia Moorhead def. Amalin SorajjaMacalester | 5-7, 6-3, 1-0 (10-7) |
| Round of 64 | Autumn BrunoWis.-Whitewater def. Rachel LindrudGustavus Adolphus | 6-0, 6-2 |
| Round of 64 | Sofia SackettLuther College def. Lily FredericksCollege of Saint Benedict | 6-2, 6-2 |
| Round of 64 | Tasha BaileyUW-La Crosse def. Maya CallawayUW-Superior | 6-0, 6-0 |
| Round of 64 | L Anna WanekSimpson def. Julia MaierSt. Scholastica | 6-2, 6-0 |
| Round of 64 | Kelsey DorrSt. Catherine def. Sydney RottBethel (MN) | 6-3, 6-2 |
| Round of 64 | Shelby CookLuther College def. Renata HernandezGustavus Adolphus | 6-1, 6-3 |
| Round of 64 | Anna StutzGustavus Adolphus def. Meadow LaDukeWartburg | 6-1, 6-1 |
| Round of 64 | Erin JarvisGrinnell College def. Sam RamseyCarthage College | 6-0, 7-5 |
| Round of 64 | Sabrina TangGrinnell College def. Ashley LaBelleCarroll (WI) | 6-0, 6-0 |
| Round of 64 | Madison SchultzCoe def. Cheyanne PhillipsConcordia Wisconsin | 6-4, 6-0 |
| Round of 64 | Cassie LeeWis.-Whitewater def. Samantha FuchsWis.-Eau Claire | 7-5, 6-3 |
| Round of 64 | Yuki OdaGustavus Adolphus def. Kaitlyn KalinkaUW-La Crosse | 6-1, 6-0 |
| Round of 64 | Halle RosentreterGustavus Adolphus def. Olivia KraftWis.-Whitewater | 6-4, 1-6, 1-0 (11-9) |
| Round of 64 | Irene AgustiGrinnell College def. Morgan NelsonWis.-Eau Claire | 6-0, 6-2 |
| Round of 64 | Madalyn BigelowWis.-Whitewater def. Alisa MakatsariaCarleton College | 6-4, 1-6, 1-0 (10-8) |
| Round of 64 | Giana ApostoliCarthage College def. Alli LauxGustavus Adolphus | 6-4, 5-7, 1-0 (11-9) |
| Round of 64 | Natalie PetersonSaint Mary's University def. Jenna ForknellConcordia Moorhead | 6-4, 5-7, 1-0 (10-2) |
| Round of 64 | Ashley TarrollyCollege of Saint Benedict def. Elaine FrantaWis.-Eau Claire | 6-0, 6-2 |
| Round of 64 | Utsah KalraGrinnell College def. Reilee SchepperSt. Catherine | 6-0, 6-0 |
| Round of 64 | Kimberly SteinertUW-La Crosse def. Hayley TrebilGustavus Adolphus | 6-2, 6-1 |
| Round of 64 | Mira ManchandaGrinnell College def. Sydney PreslerWis.-Eau Claire | 6-0, 6-0 |
| Round of 64 | Gianna NikoleitWis.-Whitewater def. Megan KarrowGustavus Adolphus | 6-4, 7-5 |
| Round of 64 | Ashley SamutaConcordia Wisconsin def. Sasha ManojlovicCarthage College | 6-0, 6-1 |
| Round of 64 | Simona PotockovaGustavus Adolphus def. Sophia de los ReyesGrinnell College | 6-1, 7-6 (7-1) |
| Round of 64 | Natalia SmithLuther College def. Maya GaedtkeCarroll (WI) | 6-3, 6-4 |
| Round of 64 | Sydney EllisonMacalester def. Sofia WalkerSt. Catherine | 6-2, 6-2 |
| Round of 64 | Clare PalenWis.-Eau Claire def. Emily KingSimpson | 6-2, 6-0 |
| Round of 64 | Mary Frances HoseCarleton College def. Maggie FryeSaint Mary's University | 6-0, 6-0 |
| Round of 32 | Grace RiermannBethel (MN) def. Cecile FuchsEdgewood College | 6-2, 6-1 |
| Round of 32 | Ioanna GiannakouGrinnell College def. Raquel EggeConcordia Moorhead | 6-1, 6-4 |
| Round of 32 | Autumn BrunoWis.-Whitewater def. Sofia SackettLuther College | 6-3, 6-1 |
| Round of 32 | Tasha BaileyUW-La Crosse def. Anna WanekSimpson | 7-5, 6-3 |
| Round of 32 | Kelsey DorrSt. Catherine def. Shelby CookLuther College | 6-1, 6-3 |
| Round of 32 | Anna StutzGustavus Adolphus def. Erin JarvisGrinnell College | 6-4, 6-2 |
| Round of 32 | Sabrina TangGrinnell College def. Madison SchultzCoe | 6-2, 6-0 |
| Round of 32 | Cassie LeeWis.-Whitewater def. Yuki OdaGustavus Adolphus | 6-2, 6-3 |
| Round of 32 | Irene AgustiGrinnell College def. Halle RosentreterGustavus Adolphus | 6-0, 6-2 |
| Round of 32 | Giana ApostoliCarthage College def. Madalyn BigelowWis.-Whitewater | 6-2, 6-4 |
| Round of 32 | Ashley TarrollyCollege of Saint Benedict def. Natalie PetersonSaint Mary's University | 6-3, 6-3 |
| Round of 32 | Kimberly SteinertUW-La Crosse def. Utsah KalraGrinnell College | 3-6, 6-3, 1-0 (10-0) |
| Round of 32 | Gianna NikoleitWis.-Whitewater def. Mira ManchandaGrinnell College | 7-6 (9-7), 6-1 |
| Round of 32 | Simona PotockovaGustavus Adolphus def. Ashley SamutaConcordia Wisconsin | 6-1, 6-0 |
| Round of 32 | Natalia SmithLuther College def. Sydney EllisonMacalester | 6-1, 6-3 |
| Round of 32 | Mary Frances HoseCarleton College def. Clare PalenWis.-Eau Claire | 6-1, 6-0 |
| Round of 16 | Grace RiermannBethel (MN) def. Ioanna GiannakouGrinnell College | 6-1, 6-3 |
| Round of 16 | Autumn BrunoWis.-Whitewater def. Tasha BaileyUW-La Crosse | 6-2, 6-1 |
| Round of 16 | Kelsey DorrSt. Catherine def. Anna StutzGustavus Adolphus | 6-1, 6-0 |
| Round of 16 | Sabrina TangGrinnell College def. Cassie LeeWis.-Whitewater | 6-4, 1-6, 1-0 (10-8) |
| Round of 16 | Irene AgustiGrinnell College def. Giana ApostoliCarthage College | 6-4, 6-1 |
| Round of 16 | Kimberly SteinertUW-La Crosse def. Ashley TarrollyCollege of Saint Benedict | 6-0, 6-2 |
| Round of 16 | Simona PotockovaGustavus Adolphus def. Gianna NikoleitWis.-Whitewater | 6-4, 1-6, 1-0 (10-6) |
| Round of 16 | Mary Frances HoseCarleton College def. Natalia SmithLuther College | 6-1, 6-2 |
| Quarterfinal | Autumn BrunoWis.-Whitewater def. Grace RiermannBethel (MN) | 6-4, 6-3 |
| Quarterfinal | Sabrina TangGrinnell College def. Kelsey DorrSt. Catherine | 6-1, 6-1 |
| Quarterfinal | Irene AgustiGrinnell College def. Kimberly SteinertUW-La Crosse | 6-4, 4-6, 6-2 |
| Quarterfinal | Mary Frances HoseCarleton College def. Simona PotockovaGustavus Adolphus | 6-0, 6-3 |
| Semifinal | Sabrina TangGrinnell College def. Autumn BrunoWis.-Whitewater | 6-1, 6-1 |
| Semifinal | Mary Frances HoseCarleton College def. Irene AgustiGrinnell College | 6-2, 5-7, 6-3 |
| Final | Sabrina TangGrinnell College def. Mary Frances HoseCarleton College | by default |
| Consolation A vs. | no result |
| Consolation round of 32 A | Peyton BuermanSaint Mary's University def. Jennifer LarsonCentral | 8-5 |
| Consolation round of 32 A | Rylee SchurmanBethel (MN) def. Rose HanGustavus Adolphus | 8-0 |
| Consolation round of 32 A | Sydney PreslerWis.-Eau Claire def. Ava HelmerHamline | 8-0 |
| Consolation round of 16 A | Sydney DouglasGustavus Adolphus def. Peyton BuermanSaint Mary's University | 8-0 |
| Consolation round of 16 A | Susi HuUW-La Crosse def. Grace BuswellNorthwestern (MN) | 8-0 |
| Consolation round of 16 A | Maya KunzeConcordia Wisconsin def. Sydney HubbardHamline | 8-3 |
| Consolation round of 16 A | Rylee SchurmanBethel (MN) def. Leah MillerWis.-Eau Claire | 8-6 |
| Consolation round of 16 A | Sydney PreslerWis.-Eau Claire def. Taylor HopkinsHamline | 8-0 |
| Consolation round of 16 A | Katie LarsonCentral def. Rachel FanciulloGustavus Adolphus | 8-5 |
| Consolation round of 16 A | Rachel PappCarroll (WI) def. Sofia HolmesSaint Mary's University | 8-3 |
| Consolation round of 16 A | Cheyanne PhillipsConcordia Wisconsin def. Emma TholeBethel (MN) | 8-1 |
| Consolation quarterfinal A | Sydney DouglasGustavus Adolphus def. Susi HuUW-La Crosse | 8-2 |
| Consolation quarterfinal A | Rylee SchurmanBethel (MN) def. Maya KunzeConcordia Wisconsin | 8-7 (8-6) |
| Consolation quarterfinal A | Sydney PreslerWis.-Eau Claire def. Katie LarsonCentral | 8-6 |
| Consolation quarterfinal A | Cheyanne PhillipsConcordia Wisconsin def. Rachel PappCarroll (WI) | 8-0 |
| Consolation semifinal A | Rylee SchurmanBethel (MN) def. Sydney DouglasGustavus Adolphus | 8-6 |
| Consolation semifinal A | Cheyanne PhillipsConcordia Wisconsin def. Sydney PreslerWis.-Eau Claire | 8-6 |
| Consolation final A | Cheyanne PhillipsConcordia Wisconsin def. Rylee SchurmanBethel (MN) | 8-5 |
| Consolation B vs. | no result |
| Consolation round of 32 B | Jersi McAlpineHamline def. Kara HandSaint Mary's University | 8-0 |
| Consolation round of 32 B | Karina MandersEdgewood College def. Ashley BarkerGustavus Adolphus | 8-1 |
| Consolation round of 32 B | Bronwyn MetcalfCentral def. Cady PetersonBethel (MN) | 8-2 |
| Consolation round of 16 B | Samantha FuchsWis.-Eau Claire def. Jersi McAlpineHamline | 8-1 |
| Consolation round of 16 B | Sarah SchirmCentral def. Karina MandersEdgewood College | 8-1 |
| Consolation round of 16 B | Sara BorchardGustavus Adolphus def. Reyna BaumgarnNorthwestern (MN) | 8-0 |
| Consolation round of 16 B | Elise BierbaumBethel (MN) def. Ella BoomgaardenSaint Mary's University | 8-0 |
| Consolation round of 16 B | Ally VerbauwhedeEdgewood College def. Elise JarchowNorthwestern (MN) | 8-5 |
| Consolation round of 16 B | Ashley RenwickSaint Mary's University def. Karen FrerkingConcordia Wisconsin | 8-7 (7-3) |
| Consolation round of 16 B | Michaela PintoCoe def. Luna van TolLuther College | 8-6 |
| Consolation round of 16 B | Bronwyn MetcalfCentral def. Emily NormanGustavus Adolphus | 8-7 (7-2) |
| Consolation quarterfinal B | Samantha FuchsWis.-Eau Claire def. Sarah SchirmCentral | 8-0 |
| Consolation quarterfinal B | Elise BierbaumBethel (MN) def. Sara BorchardGustavus Adolphus | 8-3 |
| Consolation quarterfinal B | Ally VerbauwhedeEdgewood College def. Ashley RenwickSaint Mary's University | 8-5 |
| Consolation quarterfinal B | Bronwyn MetcalfCentral def. Michaela PintoCoe | 8-2 |
| Consolation semifinal B | Samantha FuchsWis.-Eau Claire def. Elise BierbaumBethel (MN) | 8-2 |
| Consolation semifinal B | Bronwyn MetcalfCentral def. Ally VerbauwhedeEdgewood College | 8-2 |
| Consolation final B | Samantha FuchsWis.-Eau Claire def. Bronwyn MetcalfCentral | 8-3 |
| Consolation A vs. B vs. | no result |
| Consolation final | Samantha FuchsWis.-Eau Claire def. Cheyanne PhillipsConcordia Wisconsin | 8-2 |
