| Singles A vs. | no result |
| A16 | Taylor HopkinsHamline def. Kallie OlsonUW-Superior | 6-0, 6-3 |
| A16 | Belinda WeddleNorthwestern (MN) def. Abby WestrumConcordia Moorhead | 6-2, 6-3 |
| A16 | Emily HendersonSaint Mary's University def. Chihiro OnoWartburg | 3-6, 6-1, 1-0 (11-9) |
| A16 | Mia AndraeConcordia Wisconsin def. Sophie GeskeWis.-Eau Claire | 6-0, 3-6, 1-0 (10-7) |
| A16 | Luna van TolLuther College def. Ashley BarkerGustavus Adolphus | 6-0, 6-1 |
| A16 | Rayna BaumgarnNorthwestern (MN) def. Alina TweetEdgewood College | 6-4, 4-6, 1-0 (12-10) |
| A8 | Bryce BigelowWis.-Whitewater def. Taylor HopkinsHamline | 6-4, 6-2 |
| A8 | Emily HendersonSaint Mary's University def. Belinda WeddleNorthwestern (MN) | 7-6 (7-0), 6-4 |
| A8 | Mia AndraeConcordia Wisconsin def. Luna van TolLuther College | 6-3, 7-5 |
| A8 | Emily CarlsonGustavus Adolphus def. Rayna BaumgarnNorthwestern (MN) | 6-1, 6-1 |
| A4 | Bryce BigelowWis.-Whitewater def. Emily HendersonSaint Mary's University | 6-3, 6-2 |
| A4 | Emily CarlsonGustavus Adolphus def. Mia AndraeConcordia Wisconsin | 7-6 (7-1), 6-1 |
| A2 | Emily CarlsonGustavus Adolphus def. Bryce BigelowWis.-Whitewater | 2-6, 6-3, 1-0 (10-8) |
| Singles B vs. | no result |
| B16 | Jersi McAlpineHamline def. Jordana BurkelandConcordia Wisconsin | 6-3, 6-1 |
| B16 | L Natalie KaiserWartburg def. Julia EllefsonSt. Scholastica | 6-0, 6-0 |
| B16 | Peyton BuermanSaint Mary's University def. Taylar SmithNorthwestern (MN) | 7-6 (7-4), 1-6, 1-0 (10-6) |
| B16 | Emma LassenCoe def. Makenzie RobinsonWis.-Eau Claire | 3-6, 7-5, 1-0 (10-5) |
| B16 | Emily SavageauConcordia Moorhead def. Maggie BurgmeierEdgewood College | 4-6, 6-2, 1-0 (10-2) |
| B16 | Avery MunsterMacalester def. Madeline KleinkeUW-La Crosse | 3-6, 6-3, 1-0 (10-6) |
| B8 | Renata MartinezLuther College def. Jersi McAlpineHamline | 7-5, 6-1 |
| B8 | Natalie KaiserWartburg def. Peyton BuermanSaint Mary's University | 6-1, 6-3 |
| B8 | Emily SavageauConcordia Moorhead def. Emma LassenCoe | 4-6, 6-4, 1-0 (11-9) |
| B8 | Lili ElofsonGustavus Adolphus def. Avery MunsterMacalester | 6-1, 6-1 |
| B4 | Renata MartinezLuther College def. Natalie KaiserWartburg | 6-2, 6-0 |
| B4 | Lili ElofsonGustavus Adolphus def. Emily SavageauConcordia Moorhead | 6-2, 6-0 |
| B2 | Renata MartinezLuther College def. Lili ElofsonGustavus Adolphus | 7-5, 6-1 |
| Singles C vs. | no result |
| Consolation round of 16 | Kalea MartinGrinnell College def. Jena SmithWisconsin Lutheran | 6-0, 6-2 |
| Consolation round of 16 | Maddie DosserSaint Mary's University def. Karina MandersEdgewood College | 6-3, 7-5 |
| Consolation round of 16 | Annie OrvisNorthwestern (MN) def. Cady PetersonBethel (MN) | 6-4, 6-4 |
| Consolation round of 16 | Kiley NolanLuther College def. Peyton MorganConcordia Wisconsin | 6-2, 6-3 |
| Consolation round of 16 | Katie KearneyUW-La Crosse def. Alex SturmWisconsin Lutheran | 6-0, 6-0 |
| Consolation round of 16 | Jania Carter-JohnsonCoe def. Ava HelmerHamline | 6-1, 6-0 |
| Consolation round of 16 | Mary SkorichConcordia Moorhead def. Taylor WentlandNorthwestern (MN) | 6-1, 6-4 |
| Consolation round of 16 | Josie CarlsonGustavus Adolphus def. Shelby HansonUW-Superior | 6-1, 6-0 |
| Consolation quarterfinal | Kalea MartinGrinnell College def. Maddie DosserSaint Mary's University | 6-2, 6-0 |
| Consolation quarterfinal | Kiley NolanLuther College def. Annie OrvisNorthwestern (MN) | 6-3, 4-6, 1-0 (10-5) |
| Consolation quarterfinal | Katie KearneyUW-La Crosse def. Jania Carter-JohnsonCoe | 6-3, 6-0 |
| Consolation quarterfinal | Josie CarlsonGustavus Adolphus def. Mary SkorichConcordia Moorhead | 7-6 (7-5), 6-1 |
| Consolation semifinal | Kalea MartinGrinnell College def. Kiley NolanLuther College | 6-2, 6-4 |
| Consolation semifinal | Katie KearneyUW-La Crosse def. Josie CarlsonGustavus Adolphus | 7-6 (7-2), 6-1 |
| Consolation final | Katie KearneyUW-La Crosse def. Kalea MartinGrinnell College | 6-1, 6-1 |
| D Singles vs. | no result |
| D16 | Sydney MarinGrinnell College def. Talia ChaitMacalester | 6-0, 6-1 |
| D16 | Claudia BilkeyCoe def. Karen FrerkingConcordia Wisconsin | 6-2, 6-0 |
| D16 | Elli DodgeNorthwestern (MN) def. Mary ShogrenWis.-Eau Claire | 6-2, 6-0 |
| D16 | W Maria ValentiniSt. Scholastica def. Callie BelschnerEdgewood College | 6-2, 6-1 |
| D16 | Riley O'DonnellLuther College def. Erin BorchardConcordia Moorhead | 6-1, 6-3 |
| D16 | Macy VossBethel (MN) def. Lainey ZurnUW-Superior | 6-2, 6-1 |
| D16 | Ashley RenwickSaint Mary's University def. Caroline HutchinsonWartburg | 6-0, 3-6, 1-0 (10-4) |
| D16 | Mary EnrightGustavus Adolphus def. Ali SchmidtWisconsin Lutheran | 6-1, 6-0 |
| D8 | Sydney MarinGrinnell College def. Claudia BilkeyCoe | 6-2, 6-1 |
| D8 | W Maria ValentiniSt. Scholastica def. Elli DodgeNorthwestern (MN) | 6-3, 6-4 |
| D8 | Riley O'DonnellLuther College def. Macy VossBethel (MN) | 6-2, 6-4 |
| D8 | Mary EnrightGustavus Adolphus def. Ashley RenwickSaint Mary's University | 6-2, 6-2 |
| D4 | L Sydney MarinGrinnell College def. Maria ValentiniSt. Scholastica | 6-1, 6-1 |
| D4 | Riley O'DonnellLuther College def. Mary EnrightGustavus Adolphus | 6-3, 6-0 |
| D2 | Riley O'DonnellLuther College def. Sydney MarinGrinnell College | 3-6, 6-3, 1-0 (11-9) |
| E Doubles vs. | no result |
| E16 | Anna HackerConcordia Moorhead def. Maya KunzeConcordia Wisconsin | 6-3, 4-6, 1-0 (10-2) |
| E16 | Ambriel JacobsWartburg def. Donni KinghornSimpson | 6-0, 6-4 |
| E16 | Jenna KoeppUW Stevens Point def. Carolyn MerkatorisCarroll (WI) | 6-1, 6-1 |
| E16 | MacKenzie LindowWis.-Whitewater def. Caitlyn SwigartNorthwestern (MN) | 6-1, 6-0 |
| E16 | Katelynn HaskellWisconsin Lutheran def. Megan GrimmLuther College | 6-1, 6-4 |
| E16 | Rachel FanciulloGustavus Adolphus def. Gigi KueckerEdgewood College | 6-0, 6-1 |
| E16 | Eliza SundermannCoe def. Leah MillerWis.-Eau Claire | 6-4, 7-6 (7-4) |
| E8 | Bruna CostaLuther College def. Anna HackerConcordia Moorhead | 7-5, 7-6 (7-2) |
| E8 | Jenna KoeppUW Stevens Point def. Ambriel JacobsWartburg | 6-3, 6-1 |
| E8 | MacKenzie LindowWis.-Whitewater def. Katelynn HaskellWisconsin Lutheran | 6-2, 6-3 |
| E8 | Eliza SundermannCoe def. Rachel FanciulloGustavus Adolphus | 7-6 (7-3), 6-3 |
| E4 | Jenna KoeppUW Stevens Point def. Bruna CostaLuther College | 6-1, 6-2 |
| E4 | MacKenzie LindowWis.-Whitewater def. Eliza SundermannCoe | 7-5, 6-0 |
| E2 | Jenna KoeppUW Stevens Point def. MacKenzie LindowWis.-Whitewater | 6-4, 6-3 |
| F Singles vs. | no result |
| F16 | Tea ArmstrongWis.-Eau Claire def. Sara BorchardGustavus Adolphus | 6-2, 6-0 |
| F16 | Michaela PintoCoe def. Mya WerningConcordia Wisconsin | 6-2, 6-7 (4-7), 1-0 (10-3) |
| F16 | Emily RubbelkeLuther College def. Grace BuswellNorthwestern (MN) | 6-1, 6-1 |
| F16 | Ellie MurphyEdgewood College def. Chloe LeitholdUW Stevens Point | 4-6, 7-5, 1-0 (10-8) |
| F16 | Rachel PappCarroll (WI) def. Kennedi WrightSimpson | 6-2, 6-3 |
| F16 | Georgia NissenWartburg def. Taylor VyskocilHamline | 6-0, 6-2 |
| F8 | Tea ArmstrongWis.-Eau Claire def. Madison MagnaniWis.-Eau Claire | 6-0, 6-0 |
| F8 | Emily RubbelkeLuther College def. Michaela PintoCoe | 6-2, 6-2 |
| F8 | Ellie MurphyEdgewood College def. Rachel PappCarroll (WI) | 5-7, 6-2, 1-0 (14-12) |
| F8 | Kylie DunningLuther College def. Georgia NissenWartburg | 6-1, 6-0 |
| F4 | Tea ArmstrongWis.-Eau Claire def. Emily RubbelkeLuther College | 6-2, 3-6, 1-0 (11-9) |
| F4 | Ellie MurphyEdgewood College def. Kylie DunningLuther College | 6-3, 6-2 |
| F2 | Ellie MurphyEdgewood College def. Tea ArmstrongWis.-Eau Claire | 4-6, 6-2, 1-0 (10-3) |
| G Singles vs. | no result |
| G16 | Sarah FergusonWis.-Whitewater def. Bailey SinnwellWartburg | 6-1, 6-0 |
| G16 | Makaylah LegelBethel (MN) def. Ana MarquardtNorthwestern (MN) | 6-4, 7-6 (7-0) |
| G16 | Jessica DahlLuther College def. Avery NicholsonUW-La Crosse | 6-2, 6-2 |
| G16 | Maddy CoopmansEdgewood College def. Elizabeth JohnsonConcordia Wisconsin | 6-7 (4-7), 6-2, 1-0 (10-3) |
| G16 | Lexi HastingsLuther College def. Francesca GrimesCarroll (WI) | 7-6 (8-6), 1-6, 1-0 (10-7) |
| G16 | Lily VangHamline def. Rose HanGustavus Adolphus | 6-1, 6-4 |
| G16 | Bella TranquilinoSimpson def. Chiyono OwaUW-Superior | 6-1, 6-3 |
| G16 | Morgan DekanWis.-Eau Claire def. Angelina GuevaraCoe | 6-4, 6-3 |
| G8 | Sarah FergusonWis.-Whitewater def. Makaylah LegelBethel (MN) | 6-1, 6-2 |
| G8 | Jessica DahlLuther College def. Maddy CoopmansEdgewood College | 7-5, 6-4 |
| G8 | Lexi HastingsLuther College def. Lily VangHamline | 6-3, 6-4 |
| G8 | Morgan DekanWis.-Eau Claire def. Bella TranquilinoSimpson | 6-1, 6-0 |
| G4 | Sarah FergusonWis.-Whitewater def. Jessica DahlLuther College | 6-3, 6-3 |
| G4 | Lexi HastingsLuther College def. Morgan DekanWis.-Eau Claire | 6-4, 6-3 |
| G2 | Lexi HastingsLuther College def. Sarah FergusonWis.-Whitewater | 7-6 (7-2), 6-0 |
| H Singles vs. | no result |
| H16 | Gabby FlynnWartburg def. Sydney HubbardHamline | 6-0, 7-6 (7-2) |
| H16 | Jaden FuquaCoe def. Auden PearsonConcordia Wisconsin | 6-1, 6-1 |
| H16 | Madison OstranderCarroll (WI) def. Jalen DenHartogLuther College | 7-6 (7-2), 6-0 |
| H16 | Sophia KniggeUW-La Crosse def. Maeryn ShepherdEdgewood College | 6-0, 6-0 |
| H16 | Elise JachowNorthwestern (MN) def. Natalie GirardWis.-Eau Claire | 6-4, 6-4 |
| H8 | M. Ramirez-MainouLuther College def. Gabby FlynnWartburg | 6-2, 6-2 |
| H8 | Faith LeitholdUW Stevens Point def. Jaden FuquaCoe | 6-2, 6-3 |
| H8 | Madison OstranderCarroll (WI) def. Sophia KniggeUW-La Crosse | 6-3, 6-3 |
| H8 | Brooke HaddorffGustavus Adolphus def. Elise JachowNorthwestern (MN) | 6-1, 6-3 |
| H4 | Faith LeitholdUW Stevens Point def. M. Ramirez-MainouLuther College | 6-2, 6-1 |
| H4 | Brooke HaddorffGustavus Adolphus def. Madison OstranderCarroll (WI) | 6-3, 6-3 |
| H2 | Faith LeitholdUW Stevens Point def. Brooke HaddorffGustavus Adolphus | 6-4, 6-3 |
